Control of Hypocotyl Elongation by Blue Light in Arabidopsis Seedlings

蓝光对拟南芥幼苗下胚轴伸长的控制

• 批准号: 9974585
• 负责人: Edgar Spalding
• 金额: $ 30万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2002-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9974585&HistoricalAwards=false
• 关键词: Control; Hypocotyl; Elongation; Blue; Light

A detailed description of the mechanisms plants use to sense and respond to their light environment is necessary for a complete understanding of plant development, which is important to those concerned with the practical uses of plants in agriculture and biotechnology. The activities funded by this award will provide molecular-level details about how the blue region of the light spectrum exerts its profound effects on Arabidopsis seedling growth and development. The mutants to be employed have lesions in genes that are believed to encode light receptors, such as the cryptochromes (CRY1 and CRY2) and photokinase (NPH1). By measuring the growth responses to blue light of the mutant seedlings with high resolution, and comparing them to the wild type, details will be learned about how the photoreception mechanism operates. Plants containing mutations in more than one photoreceptor will also be created and studied. This comparative kinetic analysis of the growth responses to blue light will indicate when a particular photoreceptor begins to exert its effect. Such information on timing will enable a very focused genomics-based search for genes that are turned on or off by biochemical pathways emanating from the various genetically-identified blue light receptors to cause the growth responses. This aspect of the project will employ microarrays of DNA on chips that represent the Arabidopsis genome. Once such blue-light regulated genes are identified, studies of their properties and functions will be initiated so that the signaling pathway becomes known at the molecular level. It is expected that the molecular details of light-signaling pathways will be an important component of the knowledge base that will support plant technologies of the future. The research supported by this award will also impact education through the involvement of postdoctoral, graduate, and undergraduate students.

详细描述植物用来感知和响应光环境的机制对于全面了解植物的发育是必要的，这对那些关注植物在农业和生物技术中的实际应用的人来说是重要的。该奖项资助的活动将提供分子水平的细节，说明光谱的蓝色区域如何对拟南芥幼苗的生长和发育产生深刻影响。将要使用的突变体在被认为编码光受体的基因中存在损伤，例如隐花色素(CRY1和CRY2)和光激酶(NPH1)。通过高分辨率测量突变体幼苗对蓝光的生长反应，并将其与野生型进行比较，将了解有关光接收机制的详细信息。含有不止一个感光器突变的植物也将被创造和研究。这种对蓝光的生长反应的比较动力学分析将表明特定的光感受器何时开始发挥其作用。这些关于时间的信息将使基于基因组学的非常专注的基因搜索成为可能，这些基因是由各种经基因识别的蓝光受体发出的生化途径开启或关闭的，从而导致生长反应。该项目的这一方面将在代表拟南芥基因组的芯片上使用DNA微阵列。一旦确定了这种蓝光调控基因，就会开始研究它们的性质和功能，以便在分子水平上了解信号通路。预计光信号通路的分子细节将成为支持未来植物技术的知识库的重要组成部分。该奖项支持的研究也将通过博士后、研究生和本科生的参与来影响教育。