Cortical Microtubule Patterning in the Arabidopsis Hypocotyl

拟南芥下胚轴的皮质微管模式

• 批准号: 1615907
• 负责人: Sidney Shaw
• 金额: $ 67.25万
• 依托单位: Indiana University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-15 至 2021-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1615907&HistoricalAwards=false
• 关键词: Cortical; Microtubule; Patterning; Arabidopsis; Hypocotyl

Plants adjust their growth plan to maximize exposure to resources. A central tool in this adaptive program is the rapid extension of stems and roots. Plants grow toward the sun or toward richer soils through careful re-orientation of their apical meristems, the stem-cell generating portions of the plant. This project focuses on the fundamental properties of cellular growth processes critical to morphogenesis. Using genetic analysis, computational models, and leading-edge microscopy techniques, this work will reveal how the internal polymer systems in the cell spatially organize in response to plant hormones to direct axial cell growth.The microtubule cytoskeleton has a fundamental role in orchestrating the axial morphogenesis of plants through its ability to influence the mechanical properties of the cell wall. Microtubules organize into patterns just beneath the cell membrane that determine where cellulose microfibrils are deposited into the cell wall. By creating co-oriented cellulose patterns, the microtubules bias cells toward axial extension. The goal of the project is to discover the genes and molecular mechanism that lead to specific microtubule patterns and the transfer of that information to the cell wall. To accomplish that goal, genetic screening and cell biological tools will be combined to determine how plant hormone receptors trigger microtubule array patterning and how the patterning of the microtubules is integrated with cell expansion.

植物调整其生长计划，以最大限度地暴露于资源。这种适应性计划的核心工具是茎和根的快速延伸。 植物通过其顶端分生组织（植物的干细胞生成部分）的仔细重新定向，朝着阳光或更肥沃的土壤生长。 这个项目的重点是细胞生长过程的基本特性，形态发生的关键。 利用遗传分析，计算模型和先进的显微镜技术，这项工作将揭示如何在细胞内的内部聚合物系统的空间组织响应植物激素，以指导轴向细胞growth.The微管细胞骨架有一个基本的作用，通过其影响细胞壁的机械性能的能力，在协调植物的轴向形态建成。微管在细胞膜下组织成图案，决定纤维素微纤维沉积到细胞壁中的位置。通过创造共同取向的纤维素模式，微管使细胞偏向轴向延伸。该项目的目标是发现导致特定微管模式的基因和分子机制，并将该信息传递到细胞壁。为了实现这一目标，遗传筛选和细胞生物学工具将结合起来，以确定植物激素受体如何触发微管阵列图案化，以及微管的图案化如何与细胞扩增相结合。

项目成果

Update: Plant Cortical Microtubule Arrays

• DOI: 10.1104/pp.17.01329
• 发表时间: 2018-01-01
• 期刊: PLANT PHYSIOLOGY
• 影响因子: 7.4
• 作者: Elliott, Andrew;Shaw, Sidney L.
• 通讯作者: Shaw, Sidney L.

CLASP Facilitates Transitions between Cortical Microtubule Array Patterns

CLASP 促进皮质微管阵列模式之间的转换

• DOI: 10.1104/pp.18.00961
• 发表时间: 2018
• 期刊: Plant Physiology
• 影响因子: 7.4
• 作者: Thoms, David;Vineyard, Laura;Elliott, Andrew;Shaw, Sidney L.
• 通讯作者: Shaw, Sidney L.

A Cycloheximide-Sensitive Step in Transverse Microtubule Array Patterning

• DOI: 10.1104/pp.18.00672
• 发表时间: 2018-10-01
• 期刊: PLANT PHYSIOLOGY
• 影响因子: 7.4
• 作者: Elliott, Andrew;Shaw, Sidney L.
• 通讯作者: Shaw, Sidney L.

Exogenous Auxin Induces Transverse Microtubule Arrays Through TRANSPORT INHIBITOR RESPONSE1/AUXIN SIGNALING F-BOX Receptors

• DOI: 10.1104/pp.19.00928
• 发表时间: 2020-02-01
• 期刊: PLANT PHYSIOLOGY
• 影响因子: 7.4
• 作者: True, Jillian H.;Shaw, Sidney L.
• 通讯作者: Shaw, Sidney L.