SGER: Biomechanical Regulation of Architecture of Engineered Cartilage

SGER：工程软骨结构的生物力学调节

• 批准号: 9979255
• 负责人: Robert Sah
• 金额: $ 5万
• 依托单位: University of California-San Diego
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9979255&HistoricalAwards=false
• 关键词: SGER; Biomechanical; Regulation; Architecture; Engineered

9979255SahThere is a need to extend the scientific basis for tissue engineering of articular cartilage for use in the replacement of arthritic joints. Normally, articular cartilage forms under the influence of mechanical load. Currently, the in vitro synthesis of cartilaginous tissue constructs from isolated chondrocytes is done in the absence of load, requires prolonged culture (for many months), and does not achieve a tissue with the composition, structure, and function of normal articular cartilage. The immediate aims of this project are to establish the feasibility of applying dynamic loading during construct growth in order to modulate tissue formation and of developing an associated theoretical framework. The specific aims are:(1) To develop a method for synthesizing three-dimensional cartilaginous tissue constructs from isolated chondrocytes in a manner that allows application of compressive load at defined amplitude, frequency, and duration, and to use this method to evaluate construct formation with certain cell densities and loading conditions.(2) To develop a simple theoretical model that describes the time-course of evolution of tissue composition and cumulative release into the medium of tissue products based on parameters that can be measured in short-term experiments, and to determine the sensitivity of the model to these parameters and the variability in the measured values.The proposed work will emphasize quantitative aspects of tissue engineering. During the course of the study, human resources will be developed for tissue engineering through the training one post-doctoral fellow and one graduate student.

需要扩展用于关节炎关节置换的关节软骨组织工程的科学基础。通常，关节软骨在机械负荷的影响下形成。目前，从分离的软骨细胞体外合成软骨组织构建体是在没有负荷的情况下进行的，需要长时间的培养（数月），并且不能获得具有正常关节软骨的组成、结构和功能的组织。该项目的直接目标是建立在结构生长过程中施加动态载荷的可行性，以调节组织形成和开发相关的理论框架。具体目的是：（1）开发一种方法，用于从分离的软骨细胞合成三维软骨组织构建体，其方式允许以限定的振幅、频率和持续时间施加压缩载荷，并使用该方法评估具有一定细胞密度和载荷条件的构建体形成。(2)为了建立一个简单的理论模型，描述组织成分的演变过程和累积释放到介质中的组织产品的参数，可以在短期实验的基础上，并确定这些参数和测量值的变化模型的灵敏度，拟议的工作将强调定量方面的组织工程。在研究过程中，将通过培养一名博士后和一名研究生来开发组织工程的人力资源。