Electrokinetic Flow Design for Micro-Laboratories on a Chip

这个工程微系统：“XYZ”芯片项目的目标是在微观尺度上研究生化分析。这笔资金将用于支持圣母大学和Caliper技术公司之间的合作项目，该项目将开发用于对纳升大小的样品进行快速、控制良好、低试剂生化分析的芯片。这种芯片的应用包括药物的快速组合筛选，温度和浓度依赖性反应动力学的测量，以及基因分型和筛选。这些应用是通过电渗透通过微蚀刻通道移动液体样品来执行的。虽然流体流动控制，如样品稀释、加热/冷却、流混合和划分已经成功证明，但有几个问题限制了吞吐量，并阻碍了在芯片上实现多功能、集成、计算机控制、网络化生化实验室。为了充分实现芯片上实验室的潜力，需要解决的具体问题是：通过通道建模减少色散，通过通道建模和建模增强色散，增强传热，以及通过封装进行溶质取样。所有的设计都将在实际制造的芯片上进行测试，与卡钳技术公司合作。

The goal of this Engineering Microsystems: "XYZ" on a Chip project is to investigate biochemical assays on a microscale. Funds are awarded to support a collaborative project between Notre Dame University and Caliper Technologies to develop chips for rapid, well controlled, low-reagent biochemical assays on nanoliter-sized samples. Applications of such chips include rapid combinatorial screening of pharmaceuticals, measurements of temperature and concentration-dependent reaction kinetics, and genetic typing and screening. These applications are executed by moving liquid samples through micro-etched channels through electro-osmosis. While fluid flow controls such as sample dilution, heating/cooling, stream mixing, and partitioning have been demonstrated successfully, there are several issues that limit the throughput and prevent the full realization of a multi-function, integrated, computer controlled, networked biochemical laboratory on a chip. Specific issues which need to be addressed to fully realize the potential of the laboratory on a chip are: dispersion reduction through channel profiling, dispersion enhancement through channel patterning and profiling, enhanced heat transfer, and solute sampling via encapsulation. All designs will be tested on actual fabricated chips in collaboration with Caliper Technologies.