I-Corps: A Low-Cost, Rapid, Sensitive, PCR-Free Pathogen Diagnostics Platform

I-Corps：低成本、快速、灵敏、无 PCR 的病原体诊断平台

• 批准号: 1313543
• 负责人: Hsueh-Chia Chang
• 金额: $ 5万
• 依托单位: University of Notre Dame
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-01-15 至 2013-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1313543&HistoricalAwards=false
• 关键词: Corps; Low; Cost; Rapid; Sensitive

Portable and multi-target DNA/RNA diagnostics requires a rapid, field-usable, simple to operate, regenerable and economical biosensor system. The nanomembrane technology presented here is based upon the integration of newly discovered electrokinetic phenomena in nanoporous membranes that promise to extend traditional label-free electrochemical and capacitance/conductance biosensors to the requisite robustness, sensitivity, selectivity and assay speed for on-field nucleic acid detection. The proposed work will optimize and integrate these new physical phenomena into an automated membrane sensing platform for hand-held DNA/RNA devices suitable for field applications by scientifically scrutinizing the detailed non-equilibrium electrokinetic phenomena and by exploiting the latest nano/microfabrication technologies. The proposed work will heavily involve development of miniature prototypes that can have a significant impact on biological research and, more commercially, the biotechnology industry sector. A viable portable and label-free DNA/RNA detection platform for bacterial detection has the potential to spur a technological advance in the biosensor industry, as it could transform pathogen detection methodology for medical, environmental, and agricultural applications by eliminating the time-consuming nucleic acid amplification step and the costly/bulky/personnel-intensive optical detectors of fluorescent sensing. The project can hence impact both nanoscience and nano biotechnology. The flexibility of the platform to test various pathogenic targets related to health and environmental safety could vastly increase the target portfolio and widen the impact of the technology.

便携式和多目标 DNA/RNA 诊断需要快速、可现场使用、操作简单、可再生且经济的生物传感器系统。这里介绍的纳米膜技术基于纳米多孔膜中新发现的动电现象的集成，有望将传统的无标记电化学和电容/电导生物传感器扩展到现场核酸检测所需的稳健性、灵敏度、选择性和测定速度。拟议的工作将通过科学地审查详细的非平衡动电现象并利用最新的纳米/微加工技术，将这些新的物理现象优化并集成到适合现场应用的手持式 DNA/RNA 设备的自动化膜传感平台中。拟议的工作将主要涉及微型原型的开发，这可能对生物研究以及更商业化的生物技术行业产生重大影响。用于细菌检测的可行的便携式、无标记的DNA/RNA检测平台有可能推动生物传感器行业的技术进步，因为它可以通过消除耗时的核酸扩增步骤和昂贵/笨重/人员密集的荧光传感光学检测器来改变医疗、环境和农业应用的病原体检测方法。因此，该项目可以影响纳米科学和纳米生物技术。该平台测试与健康和环境安全相关的各种致病目标的灵活性可以极大地增加目标组合并扩大该技术的影响。