Fundamental Investigations of Electrospray Ionization Mass Spectrometry

这项题为“电喷雾电离质谱法的基础研究”的研究是新奥尔良大学的Richard科尔博士和泽维尔大学（HBCU）的王光迪博士合作进行的，重点是与电喷雾电离相关的基础研究过程。 这项研究将包括一个独特的电化学电池内的电喷雾探针的发展，并将调查电喷雾电离增强氯化物附着。 这项研究有三个科学目标。 首先，一种新的电化学/电喷雾池将用于研究电化学产生的反应产物和中间体在真实的时间。 电池性能将通过与已知的、充分表征的系统的实验结果的比较来验证。 将测试的两个系统包括多环芳烃（PAH）与DNA和邻苯二酚胺与亲核试剂的反应。 第二个目标是映射存在于作为电喷雾过程的一部分形成的泰勒锥中的电势梯度。 这一基础研究可能会导致界面设计的改进，这将显着提高毛细管电泳（CE）耦合到液相色谱/质谱（LC/MS）。 第三项研究将详细研究使用氯离子附着作为一种手段，以提高电喷雾LC/MS中形成的负离子的灵敏度和结构信息。 这项研究将导致更清楚地了解电离过程，这可能导致新的仪器和新的分析程序。 此外，这种研究合作将大大提高少数民族参与分析科学。

The research entitled "Fundamental Investigations of Electrospray Ionization Mass Spectrometry" is a collaboration between Dr. Richard Cole at the University of New Orleans and Dr. Guangdi Wang at Xavier University (an HBCU) and focuses on fundamental studies related to the electrospray ionization process. The study will include the development of a unique electrochemical cell inside of an electrospray probe, and will investigate electrospray ionization enhancements provided by chloride attachment. There are three scientific objectives to this research. First, a novel electrochemical/electrospray cell will be used to study electrochemically generated reaction products and intermediates in real time. The cell performance will be validated by comparison of the experimental results with known, well characterized systems. Two systems which will be tested include the reactions of polyaromatic hydrocarbons (PAH's) with DNA and catecholamines with nucleophiles. The second goal is to map the potential gradient existing in the Taylor cone formed as part of the electrospray process. This fundamental study could result in improvements to interface design which will significantly enhance capillary electrophoresis (CE) coupled to liquid chromatography/mass spectrometry (LC/MS). The third study will examine, in detail, the use of chloride ion attachment as a means to enhance sensitivity and structural information about negative ions formed in electrospray LC/MS.The results of this study could potentially impact both electrochemical science and mass spectrometry. This research will lead to a clearer understanding of the ionization process which may result in new instrumentation and new analytical procedures. Additionally, this research collaboration will greatly enhance minority participation in the analytical sciences.