Fundamental Investigations of Electrospray Mass Spectrometry

理查德教授B。新奥尔良大学的科尔和王光迪在化学系分析和表面化学项目的支持下：a）表征环境反应性气体对质谱中电喷雾发射器内电势分布的影响; B）获取和评估一系列完整的电流测量，以提高对电化学反应在功能中发挥的重要作用的理解，电喷雾装置的成功; c）开发快速电化学/电喷雾质谱技术，以研究无法通过其他手段获得的电化学反应机制;和d）开发一种对电喷雾生成的络合物进行热化学测量的新方法，并将其应用于测量锂离子加合物的键离解能，和分子量在300-700道尔顿范围内的脂质的卤化物加合物电喷雾源的低离子产率是更好的质谱分析的主要障碍。 各种分子种类的离子形成机制尚不清楚。这项研究将推进我们对电喷雾发射器内的电化学反应阵列及其可能发生的速率的理解。 对电喷雾的这一固有方面的详细理解增强了分析物的电离，并改进了它们的检测和质谱分析。 该项目包括新奥尔良大学和路易斯安那州泽维尔大学（HBCU）之间的合作伙伴关系计划，以促进这两个机构的学生接触先进的概念和方法质谱。

Professors Richard B. Cole and Guangdi Wang of the University of New Orleans are supported by the Analytical and Surface Chemistry Program in the Division of Chemistry to: a) characterize the effect of ambient reactive gases on potential distributions within the electrospray emitter in mass spectrometry; b) acquire and evaluate a full array of current measurements to improve the understanding of the essential role that electrochemical reactions play in the functioning and success of electrospray device; c) develop a fast electrochemistry / electrospray mass spectrometry technique to investigate electrochemical reaction mechanisms that are not accessible by other means; and d) develop a new method to make thermochemical measurements on electrospray-generated complexes, and apply it to measure bond dissociation energies of lithium ion adducts, and halide adducts of lipids having molecular weights in the range of 300-700 DaltonsThe low ion yield from electrospray sources is a major hindrance to better mass spectrometric analysis. The mechanism of ion formation for a wide variety of molecular species is not known. This research will advance our understanding of the array of electrochemical reactions within the electrospray emitter and the rates at which they may occur. Detailed understanding of this inherent aspect of electrospray enhances the ionization of analytes and improves their detection and analysis by mass spectrometry. The project includes a plan for a cooperative partnership between the University of New Orleans and Xavier University of Louisiana (an HBCU) to promote exposure of students at both institutions to advanced concepts and methodologies in mass spectrometry.