CAREER: Peptide Adsorption Dynamics on Molecularly Imprinted Surfaces: A Surface Plasmon Resonance Study

职业：分子印迹表面上的肽吸附动力学：表面等离子共振研究

• 批准号: 9983737
• 负责人: Scott Husson
• 金额: $ 49万
• 依托单位: Clemson University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-01 至 2006-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9983737&HistoricalAwards=false
• 关键词: CAREER; Peptide; Adsorption; Dynamics; Molecularly

ABSTRACT CTS-9983737 S. M. Husson Clemson U.Molecular imprinting provides a promising technique for designing materials that possess the molecular recognition properties of biological systems. This process produces a polymeric material that contains a molecular imprint of a target molecule with size and shape dimensions that match those of the target molecule. This research describes a methodology that uses surface plasmon resonance (SPR) spectroscopy to examine peptide adsorption dynamics on molecularly imprinted polymer (MIP) surfaces. The project has three primary goals: i) to synthesize uniform, ultrathin MIP films with predetermined thickness on SPR biosensor chips; ii) to measure in real time the adsorption properties of target peptides on their corresponding MIP films; and iii) to apply fundamental information gained from these measurements to design and test chromatographic separation materials for peptide separations. The first goal will be achieved by carrying out surface-confined living radical polymerization (SCLRP) on the SPR biosensor chips in the presence of target peptides. SPR spectroscopy will be used to accomplish the second goal. The third goal will be met by synthesizing our MIP films on commercially available chromatography support materials. Synthesis of an MIP film by SCLRP and subsequent use of SPR to measure the fundamental adsorption properties of these films represent the novel features of this research career plan. Results of the research will broadly impact the development of a range of MIP materials, including tailor-made separation materials, antibody and receptor binding sites mimics in recognition and assay systems, enzyme site mimics for catalysis, and recognition elements in biosensors.Elementary school teachers assigned to teach multiple subjects often cite mathematics and science as their least comfortable subjects to teach. The education plan has four primary goals: i) to teach elementary school teachers and preservice teachers that teaching mathematics and science means interaction with materials and fostering independent thinking; ii) to educate teachers about programs that employ inquiry-driven, activity-based learning; iii) to train and support teachers to implement these programs; and iv) to encourage and aid teachers to disseminate their experiences to other teachers.

摘要CTS-9983737 S.M.Husson Clemson U分子印迹技术为设计具有生物体系分子识别特性的材料提供了一种很有前途的技术。这一过程产生的聚合物材料含有目标分子的分子印迹，其尺寸和形状尺寸与目标分子的尺寸和形状尺寸相匹配。这项研究描述了一种使用表面等离子体共振(SPR)光谱来检测多肽在分子印迹聚合物(MIP)表面的吸附动力学的方法。该项目有三个主要目标：i)在SPR生物传感器芯片上合成具有预定厚度的均匀超薄MIP膜；ii)实时测量目标多肽在其相应MIP膜上的吸附性能；以及iii)将这些测量获得的基本信息应用于设计和测试用于多肽分离的色谱分离材料。第一个目标将通过在SPR生物传感器芯片上进行表面受限活性自由基聚合(SCLRP)来实现。为了实现第二个目标，将使用表面等离子体共振谱。第三个目标将通过在商业上可获得的层析支持材料上合成我们的MIP膜来实现。用SCLRP合成MIP膜，并随后使用SPR测量这些膜的基本吸附性能，代表了这一研究生涯计划的新特点。这项研究的结果将广泛影响一系列MIP材料的发展，包括定制的分离材料，识别和检测系统中的抗体和受体结合位点模拟，催化的酶位点模拟，以及生物传感器中的识别元件。被分配教授多门学科的小学教师经常将数学和科学作为他们最不舒服的科目。教育计划有四个主要目标：i)教育小学教师和职前教师，教数学和科学意味着与材料互动和培养独立思考；ii)教育教师关于采用探究驱动、基于活动的学习的计划；iii)培训和支持教师实施这些计划；以及iv)鼓励和帮助教师向其他教师传播他们的经验。