CAREER: Automated Annotation of Functional Pathways using Eukaryotic cDNA Sequence Data

表7叙述DBI 9984882 Gaasterland Rockefeller大学生涯：使用真核生物cDNA序列数据对功能通路进行自动注释该项目将建立一个系统，该系统可供生物学家在本地机器上使用或通过网络访问，以分析任意数量的新cDNA序列，将分析与微阵列表达数据相结合，并根据功能相关性组织cDNA。为了满足两个目标，我们将对组装的真核细胞的cDNA序列进行分析：1.识别相互依赖的cDNA，这些cDNA可以被注释为相同或相连的代谢和信号通路的组成部分，以及2.为了识别未知功能但具有进化或基因组特征的cDNA，这项工作将分两个阶段进行；第一阶段将基于鹊类基因组注释环境建立真核基因的功能注释系统，第二阶段将建立额外的模块来对进化模式和基因组组织进行注释后分析。该研究计划还将作为建设题为“基因组注释原理”和“生物数据库设计原理”的培训班的基础。网址：http://genomes.rockefeller.edu/magpie

Form 7 NarrativeDBI 9984882 Gaasterland Rockefeller University CAREER: Automated annotation of Functional Pathways using Eukaryotic cDNA Sequence DataThis project will build a system that can be used by biologists either on local machines or accessed over the Web to analyze arbitrarily large numbers of novel cDNA sequences, to integrate the analysis with microarray expression data and to organize cDNAs according to functional relatedness. Assembled eukaryotic cDNA sequences will be analyzed to satisfy two goals:1. To identify interdependent cDNAs that can be annotated as components of the same or connected metabolic and signaling pathways, and2. To identify cDNAs of unknown function but with evolutionary or genomic properties that indicate a relationship to known components or pathways.The work will be carried out in two phases; phase one will build a functional annotation system for eukaryotic cDNA based on the MAGPIE genome annotation environment, phase two will build additional modules to carry out post-annotation analysis of patterns of evolution and genome organization. The research program will also serve as the foundation for the construction of training courses entitled "Principles of Genome Annotation" and "Principles of Biological Database Design"URL: http://genomes.rockefeller.edu/magpie