CAREER: Automated Annotation of Functional Pathways using Eukaryotic cDNA Sequence Data

表格7叙述DBI 9984882 Gaasterland Rockefeller University职业生涯：使用真核cDNA序列数据自动注释功能途径本项目将建立一个系统，生物学家可以在本地机器上使用该系统或通过Web访问，以分析任意大量的新cDNA序列，将分析与微阵列表达数据相结合，并根据功能相关性组织cDNA。将分析组装的真核cDNA序列以满足两个目标：1.鉴定相互依赖的cDNA，这些cDNA可以被注释为相同或相关的代谢和信号传导途径的组分;为了鉴定功能未知但具有进化或基因组特性的cDNA，这些特性表明与已知组分或途径的关系。工作将分两个阶段进行：第一阶段将建立基于MAGPIE基因组注释环境的真核cDNA功能注释系统，第二阶段将建立额外的模块，以进行进化和基因组组织模式的注释后分析。该研究计划还将作为题为“基因组注释原理”和“生物数据库设计原理“的培训课程建设的基础。http://genomes.rockefeller.edu/magpie

Form 7 NarrativeDBI 9984882 Gaasterland Rockefeller University CAREER: Automated annotation of Functional Pathways using Eukaryotic cDNA Sequence DataThis project will build a system that can be used by biologists either on local machines or accessed over the Web to analyze arbitrarily large numbers of novel cDNA sequences, to integrate the analysis with microarray expression data and to organize cDNAs according to functional relatedness. Assembled eukaryotic cDNA sequences will be analyzed to satisfy two goals:1. To identify interdependent cDNAs that can be annotated as components of the same or connected metabolic and signaling pathways, and2. To identify cDNAs of unknown function but with evolutionary or genomic properties that indicate a relationship to known components or pathways.The work will be carried out in two phases; phase one will build a functional annotation system for eukaryotic cDNA based on the MAGPIE genome annotation environment, phase two will build additional modules to carry out post-annotation analysis of patterns of evolution and genome organization. The research program will also serve as the foundation for the construction of training courses entitled "Principles of Genome Annotation" and "Principles of Biological Database Design"URL: http://genomes.rockefeller.edu/magpie