CAREER: Automated Annotation of Functional Pathways using Eukaryotic cDNA Sequence Data

Gaasterland Rockefeller University职业生涯：使用真核生物cDNA序列数据自动注释功能通路这个项目将建立一个系统，生物学家可以在本地机器上使用或通过网络访问该系统来分析任意大量的新cDNA序列，将分析与微阵列表达数据集成，并根据功能相关性组织cDNA。装配的真核生物cDNA序列分析将满足两个目标：1。鉴定相互依赖的dna，这些dna可以被注释为相同或连接的代谢和信号通路的组成部分；鉴定功能未知但具有与已知成分或途径相关的进化或基因组特性的cdna。这项工作将分两个阶段进行；第一阶段将构建基于MAGPIE基因组注释环境的真核生物cDNA功能注释系统，第二阶段将构建附加模块，对进化模式和基因组组织进行注释后分析。该研究计划还将作为“基因组注释原理”和“生物数据库设计原理”培训课程建设的基础（网址：http://genomes.rockefeller.edu/magpie）

Form 7 NarrativeDBI 9984882 Gaasterland Rockefeller University CAREER: Automated annotation of Functional Pathways using Eukaryotic cDNA Sequence DataThis project will build a system that can be used by biologists either on local machines or accessed over the Web to analyze arbitrarily large numbers of novel cDNA sequences, to integrate the analysis with microarray expression data and to organize cDNAs according to functional relatedness. Assembled eukaryotic cDNA sequences will be analyzed to satisfy two goals:1. To identify interdependent cDNAs that can be annotated as components of the same or connected metabolic and signaling pathways, and2. To identify cDNAs of unknown function but with evolutionary or genomic properties that indicate a relationship to known components or pathways.The work will be carried out in two phases; phase one will build a functional annotation system for eukaryotic cDNA based on the MAGPIE genome annotation environment, phase two will build additional modules to carry out post-annotation analysis of patterns of evolution and genome organization. The research program will also serve as the foundation for the construction of training courses entitled "Principles of Genome Annotation" and "Principles of Biological Database Design"URL: http://genomes.rockefeller.edu/magpie