CAREER: Regulation of Monoclonal Antibody Production in Murine Hybridomas

职业：鼠杂交瘤中单克隆抗体产生的调控

• 批准号: 9984918
• 负责人: Susan Sharfstein
• 金额: $ 21万
• 依托单位: University of Toledo
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2004-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9984918&HistoricalAwards=false
• 关键词: CAREER; Regulation; Monoclonal; Antibody; Production

The objective of this project is to investigate the mechanism by which antibody production is regulated in cultured hybridoma cells. The specific project objectives are to: (1) determine whether the increase in antibody production in response to osmotic stress is regulated by transcription, translation or post-translational processes, (2) evaluate activities of the stress-activated and mitogen-activated protein kinase cascades in response to osmotic stress, (3) establish the role of stress response proteins and chaperones in the osmotic stress response, (4) determine the role of cell cycle in osmotic stress response, and (5) use existing mathematical models of antibody synthesis to determine rate limiting steps in antibody synthesis.

本项目的目的是研究在培养的杂交瘤细胞中调节抗体产生的机制。 具体的项目目标是：（1）确定响应于渗透胁迫的抗体产生的增加是否受转录、翻译或翻译后过程的调节，（2）评价响应于渗透胁迫的胁迫活化和促分裂原活化蛋白激酶级联的活性，（3）确定胁迫应答蛋白和伴侣蛋白在渗透胁迫应答中的作用，（4）确定细胞周期在渗透应激反应中的作用，和（5）使用现有的抗体合成数学模型来确定抗体合成中的限速步骤。