POWRE: Techniques for Investigating Cytoskeletal Effects on Recombinant Protein Glycosylation

POWRE：研究细胞骨架对重组蛋白糖基化影响的技术

• 批准号: 0083203
• 负责人: Susan Sharfstein
• 金额: $ 10.49万
• 依托单位: Albany Medical College of Union University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0083203&HistoricalAwards=false
• 关键词: POWRE; Techniques; Investigating; Cytoskeletal; Effects

The goal of this project is to investigate the effects of culture conditions on recombinant protein production, specifically the effects of cell adhesion on yield and glycosylation patterns of recombinant proteins produced in Chinese hamster ovary (CHO) cells. The specific goals for the POWRE project are: (1) to develop expertise in the techniques for studying protein glycosylation including high performance liquid chromatography and gel electrophoresis; (2) to determine what probes and imaging techniques are best suited for studies of the cytoskeleton for suspension and adherent cultures of CHO cells; and (3) to obtain preliminary data analyzing the glycoforms of tissue plasminogen activator produced under the different culture conditions and determine which of the techniques for studying protein glycosylation will be best suited for future studies.

本项目的目的是研究培养条件对重组蛋白生产的影响，特别是细胞粘附对中国仓鼠卵巢（CHO）细胞中生产的重组蛋白产量和糖基化模式的影响。POWRE项目的具体目标是：（1）发展研究蛋白质糖基化的技术，包括高效液相色谱和凝胶电泳;（2）确定什么样的探针和成像技术最适合于研究CHO细胞悬浮和贴壁培养的细胞骨架;和（3）获得分析在不同培养条件下产生的组织纤溶酶原激活物的糖型的初步数据，并确定哪种用于研究蛋白质糖基化的技术最适合于未来的研究。