CAREER: An In Vivo Selection for Enzymatic Activity

该项目包括研究目标以及补充教育计划。 研究目标涉及蛋白质文库的高通量筛选技术的发展。 涉及一种独特的方法，将合成有机化学和分子遗传学结合在一起，其中可以检测键的形成或切割，因为键用于控制依赖于蛋白质二聚化的细胞读数。 这一总体战略预计将有广泛的应用，从酶学和酶的设计，功能基因组学和药物发现。有了这个职业奖，有机和大分子化学计划的有机动力学计划支持教授弗吉尼亚W。哥伦比亚大学的康沃尔语。 教授康沃尔语将设计和组装一个分子系统，以评估小蛋白质或其他小分子的潜在影响，因为他们影响一个给定的生化过程。 教育计划旨在帮助学生在日益跨学科的研究环境中取得成功。 在本科阶段，基于主题的教学模块正在被纳入传统的有机化学课程，研究生级别的研讨会系列将解决职业发展问题，从平衡工作和个人生活到演讲。

This project comprises research goals as well as a complementary education plan. The research goals involve the development of high-throughput screening techniques for libraries of proteins. A unique approach is involved that brings together synthetic organic chemistry and molecular genetics, in which bond formation or cleavage can be detected because the bond is used to control a cellular readout that depends on protein dimerization. This general strategy is anticipated to have broad applications ranging from enzymology and enzyme design to functional genomics and drug discovery.With this CAREER Award, the Organic and Macromolecular Chemistry Program's Organic Dynamics Program supports the research of Professor Virginia W. Cornish of Columbia University. Professor Cornish will design and assemble a molecular system to assess the potential effects of small proteins or other small molecules as they affect a given biochemical process. The educational plan has been designed to help students succeed in a research environment that is increasingly interdisciplinary. At the undergraduate level, topic-based teaching modules are being incorporated into the traditional organic chemistry curriculum, and a graduate-level seminar series will address career development issues ranging from balancing work and personal life to giving talks.