CAREER: Vascular Gene Therapy: Characterization and Optimization

职业：血管基因治疗：表征和优化

• 批准号: 9985012
• 负责人: Naomi Chesler
• 金额: $ 20.01万
• 依托单位: University of Vermont & State Agricultural College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-15 至 2002-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9985012&HistoricalAwards=false
• 关键词: CAREER; Vascular; Gene; Therapy; Characterization

9985012CheslerGene therapy is an exciting new modality for treating and preventing vascular disease. Engineered gene therapy delivery techniques could make this technology more affordable and thus broadly accessible to the cardiovascular patient population. By applying quantitative engineering analyses to a living, biological system, the PI hopes to further our knowledge of human vascular transport mechanisms (aim 1), optimize adenoviral gene therapy methods (aim 2), and assess the efficacy of optimized gene therapy treatment strategies in human arteries and veins (aim 3). An ex vivo vascular culture system will be used to pursue each of these goals. Ex vivo arterial and venous organ culture is an ideal experimental paradigm for investigating vascular transport phenomena under rigorous environmental and mechanical control. Further, it allows living human tissue experimentation in a three-dimensional, near-physiological environment. The first educational goal of the proposed career plan is to teach engineering students the fundamentals in the PI's area of specialization: the thermofluid sciences (aim 1). The techniques of problem-based learning and interaction with local industry will be used to achieve this goal. The second aim is to strengthen teamwork and communication skills in both graduate and undergraduate engineers (aim 2). Specifically, students will be given design projects in classes and assigned to mixed-level and mixed-gender teams. They will be given instruction in technical communication and practice by giving presentations to their peers and a lay audience (local middle-school children). The third goal of the educational program is to addresses the problem of diversity in the engineering workforce (aim 3). University-level mentoring will be frequent and informal. Through outreach and mentoring at the university, high school and middle school levels, the PI hopes to increase the number of women in engineering and in the pipeline.

9985012Chesler基因疗法是治疗和预防血管疾病的一种令人兴奋的新方式。工程基因治疗交付技术可以使这项技术更负担得起，从而广泛适用于心血管患者群体。通过将定量工程分析应用于活体生物系统，PI希望进一步了解人类血管转运机制（目标1），优化腺病毒基因治疗方法（目标2），并评估优化基因治疗策略在人类动脉和静脉中的疗效（目标3）。将使用离体血管培养系统来实现这些目标中的每一个。离体动脉和静脉器官培养是在严格的环境和机械控制下研究血管运输现象的理想实验范例。 此外，它允许在三维的、接近生理的环境中进行活体人体组织实验。拟议职业计划的第一个教育目标是向工程专业的学生传授PI专业领域的基础知识：热流体科学（目标1）。为实现这一目标，将采用以问题为基础的学习和与当地工业界互动的技术。第二个目标是加强研究生和本科生工程师的团队合作和沟通技巧（目标2）。 具体来说，学生将在课堂上获得设计项目，并分配到混合级别和混合性别的团队。他们将通过向同龄人和非专业观众（当地中学生）进行演示来获得技术交流和实践方面的指导。教育计划的第三个目标是解决工程劳动力的多样性问题（目标3）。大学一级的辅导将是经常和非正式的。 通过在大学、高中和初中一级的推广和指导，PI希望增加工程和管道中的妇女人数。