A Wide-Field Deconvolution Microscope for Cell and Developmental Biology

SackA细胞和发育生物学宽视场反褶积显微镜反褶积显微镜提供了优越的空间分辨率（特别是在高倍倍率下），是在固定细胞和活细胞中三维共定位多个荧光探针的理想选择。俄亥俄州立大学将建立一个宽视场反褶积显微镜设备。该系统将配置在蔡司Axiophot 2显微镜周围，该显微镜带有电动平台，高分辨率冷却CCD相机，以及用于图像采集和反卷积的集成软件。目前的核心用户将专注于植物、神经生物学和真菌材料的细胞和发育生物学。具体项目包括：(1)果蝇神经系统中控制细胞增殖、终末分化和轴突生长的基因的分子功能，(2)植物囊泡运输和细胞分裂的细胞生物学和调控，(3)拟南芥和曲霉中g-微管蛋白的功能和定位，(4)拟南芥中控制气孔模式和发育的基因和机制，(5)斑马鱼轴突生长的分子遗传调控，(6)植物核基质蛋白之间的功能相互作用；(7)小鼠浦肯野细胞内蛋白和mRNA定位的差异空间调控。反褶积成像很有可能促进对几种模型系统中特定蛋白质的功能和定位的理解。该设施将加强生物科学学院、植物生物技术中心和神经生物技术中心的细胞和发育生物学家之间的互动。它还将为培养博士后研究人员、研究生和本科生作出实质性贡献。

Abstract SackA Wide-Field Deconvolution Microscope for Cell and Developmental BiologyDeconvolution microscopy provides superior spatial resolution (especially at high magnification) that is ideal for co-localizing multiple fluorescent probes in fixed and living cells in three dimensions. A wide-field deconvolution microscopy facility will be established at Ohio State University. The system will be configured around a Zeiss Axiophot 2 microscope with motorized stage, a high-resolution cooled CCD camera, and integrated software for image acquisition and deconvolution. Current core users will focus on the cell and developmental biology of plant, neurobiological and fungal material. Specific projects include: (1) the molecular functions of genes controlling cell proliferation, terminal differentiation, and axonal outgrowth in the Drosophila nervous systems, (2) the cell biology and regulation of plant vesicular trafficking and cytokinesis, (3) the functions and localization of g-tubulin in Arabidopsis and Aspergillus, (4) genes and mechanisms controlling stomatal patterning and development in Arabidopsis, (5) the molecular genetic regulation of axonal outgrowth in zebrafish, (6) functional interactions between plant nuclear matrix proteins, and (7) the differential spatial regulation of protein and mRNA localization within mouse Purkinje cells.Deconvolution imaging is highly likely to advance the understanding of the function and localization of specific proteins in several model systems. This facility will enhance interactions between cell and developmental biologists in the College of Biological Sciences, the Plant Biotechnology Center and the Neurobiotechnology Center. It will also contribute substantively to the training of post-doctoral researchers, graduate students and undergraduate students.