A Wide-Field Deconvolution Microscope for Cell and Developmental Biology

用于细胞和发育生物学的宽视场反卷积显微镜反卷积显微镜提供了上级空间分辨率（特别是在高放大倍数下），是三维固定和活细胞中多个荧光探针共定位的理想选择。将在俄亥俄州州立大学建立一个宽视场去卷积显微镜设施。 该系统将围绕Zeiss Axiophot 2显微镜进行配置，该显微镜具有电动载物台、高分辨率冷却CCD相机以及用于图像采集和去卷积的集成软件。 目前的核心用户将专注于植物的细胞和发育生物学，神经生物学和真菌材料。 具体项目包括：（1）果蝇神经系统中控制细胞增殖、终末分化和轴突生长的基因的分子功能，（2）植物囊泡运输和胞质分裂的细胞生物学和调节，（3）拟南芥和曲霉属中g-微管蛋白的功能和定位，（4）拟南芥中控制气孔模式和发育的基因和机制，（5）斑马鱼轴突生长的分子遗传调控，（6）植物核基质蛋白之间的功能相互作用，（7）小鼠浦肯野细胞内蛋白质和mRNA定位的差异空间调控。 该设施将加强生物科学学院，植物生物技术中心和神经生物技术中心的细胞和发育生物学家之间的互动。 它还将为博士后研究人员、研究生和本科生的培训作出实质性贡献。

Abstract SackA Wide-Field Deconvolution Microscope for Cell and Developmental BiologyDeconvolution microscopy provides superior spatial resolution (especially at high magnification) that is ideal for co-localizing multiple fluorescent probes in fixed and living cells in three dimensions. A wide-field deconvolution microscopy facility will be established at Ohio State University. The system will be configured around a Zeiss Axiophot 2 microscope with motorized stage, a high-resolution cooled CCD camera, and integrated software for image acquisition and deconvolution. Current core users will focus on the cell and developmental biology of plant, neurobiological and fungal material. Specific projects include: (1) the molecular functions of genes controlling cell proliferation, terminal differentiation, and axonal outgrowth in the Drosophila nervous systems, (2) the cell biology and regulation of plant vesicular trafficking and cytokinesis, (3) the functions and localization of g-tubulin in Arabidopsis and Aspergillus, (4) genes and mechanisms controlling stomatal patterning and development in Arabidopsis, (5) the molecular genetic regulation of axonal outgrowth in zebrafish, (6) functional interactions between plant nuclear matrix proteins, and (7) the differential spatial regulation of protein and mRNA localization within mouse Purkinje cells.Deconvolution imaging is highly likely to advance the understanding of the function and localization of specific proteins in several model systems. This facility will enhance interactions between cell and developmental biologists in the College of Biological Sciences, the Plant Biotechnology Center and the Neurobiotechnology Center. It will also contribute substantively to the training of post-doctoral researchers, graduate students and undergraduate students.