The Pyp regulatory proteins of Yersinia enterocolitica: functional characterization and role in signal transduction and gene expression

小肠结肠炎耶尔森氏菌的 Pyp 调节蛋白：功能特征及其在信号转导和基因表达中的作用

• 批准号: 139247916
• 负责人: Privatdozent Dr. Johannes Gerhard Heusipp
• 金额: --
• 依托单位: Hochschulverwaltung
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/139247916?language=en
• 关键词: Pyp; regulatory; proteins; Yersinia; enterocolitica

During an infection of the human host, pathogenic bacteria have to sense the changing environment and react with the coordinated expression of genes that encode factors necessary for survival and virulence. We could show that the human pathogenic bacterium Yersinia enterocolitica employs the proteins PypA, PypB, and PypC to activate the transcription of the H-NS-repressed hreP gene. The HreP protease is specifically expressed during an infection and necessary for full virulence. PypB and PypC regulate additional operons coding for pili and a secretion system, respectively. While PypB and PypC are DNA binding proteins, the function of the membrane protein PypA so far remains unknown. Using molecular and genetic analyses, we will functionally characterize the Pyp proteins and study protein-DNA and protein-protein interactions. Our studies will not only result in a functional characterization of the bacterial regulators, but also shed light on signal sensing and transduction in bacterial systems related to pathogenesis.

在感染人类宿主期间，病原菌必须感知不断变化的环境，并与编码生存和毒力所需因子的基因的协调表达作出反应。我们可以证明，人类致病性小肠结肠炎耶尔森氏菌采用蛋白质PypA，PypB和PypC来激活H-NS抑制的hreP基因的转录。HreP蛋白酶在感染过程中特异性表达，并且是完全毒力所必需的。PypB和PypC分别调节编码皮利和分泌系统的另外的操纵子。虽然PypB和PypC是DNA结合蛋白，但迄今为止膜蛋白PypA的功能仍然未知。使用分子和遗传分析，我们将功能上表征Pyp蛋白质，并研究蛋白质-DNA和蛋白质-蛋白质相互作用。我们的研究不仅将导致细菌调节因子的功能特性，而且还阐明了细菌系统中与致病相关的信号传感和转导。