A Multi-user 600 MHz Nuclear Magnetic Resonance Spectrometer for Structural Biology

用于结构生物学的多用户 600 MHz 核磁共振波谱仪

• 批准号: 0070359
• 负责人: Steven Van Doren
• 金额: $ 18.5万
• 依托单位: University of Missouri-Columbia
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-15 至 2002-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0070359&HistoricalAwards=false
• 关键词: Multi; user; 600; MHz; Nuclear

Nuclear Magnetic Resonance (NMR) spectroscopy has developed into a powerful means of determining the structure and motions of proteins and nucleic acids in solution. Such molecules form the machinery and blueprints of life. NMR is a powerful method for studying how these biomolecules recognize and interact with their molecular partners from the cell. NMR is also a crucial technique for investigating atomic-resolution details of how protein chains fold up into their 3-dimensional, functional shape. Research using this 600 MHz NMR system will emphasize determination of atomic-resolution 3D protein structures. Other work will include study of the motions and molecular interactions of these proteins and study of peptide and nucleic acid structures. The new 600 MHz system will be used to study cancer and arthritis progression, hearing in the inner ear, communication within cells, development and differentiation of plants, certain RNA-catalyzed reactions in protein synthesis, protein interactions with cell membranes, and protein stability.The state-of-the art 600 MHz NMR system features a 14.1 Tesla magnetic field that is actively shielded to minimize its space requirement. It features four channels for simultaneous radiofrequency excitation of four kinds of atomic nuclei, particularly the proton, carbon, nitrogen, phosphorous, and deuterium widely important in biomolecules. To ensure clean spectra with few artifacts and to enable measurement of biomolecular diffusion, the system includes pulsed field gradients. Two probes triply tuned to proton, carbon, and nitrogen frequencies will enable characterization of proteins and nucleic acids. The small volume of one probe accommodates concentrated samples, while the large volume of the second probe accommodates samples that can only be studied in more dilute solution. The 600 MHz NMR spectrometer will be used by five Ph.D. students and five postdoctoral fellows in biomolecular NMR from four participating laboratories and three departments. Students with training in protein structure determination in solution are currently in demand, and this demand is expected to continue for many years. The 600 MHz system features improved resolution and 1.5-fold greater sensitivity than the 500 MHz spectrometer on campus, facilitating more difficult projects. This new spectrometer, in the campus-wide NMR facility at the University of Missouri-Columbia, will help structural biology contribute to the expansion of research in life sciences, with relevance ranging from medicine to agriculture.

核磁共振波谱已经发展成为一种确定蛋白质和核酸在溶液中的结构和运动的强大手段。这些分子构成了生命的机械和蓝图。核磁共振是研究这些生物分子如何从细胞中识别并与其分子伴侣相互作用的有效方法。核磁共振也是研究蛋白质链如何折叠成其三维功能形状的原子分辨率细节的关键技术。使用这种600 MHz核磁共振系统的研究将侧重于确定原子分辨率的3D蛋白质结构。其他工作将包括研究这些蛋白质的运动和分子相互作用，以及研究多肽和核酸结构。新的600 MHz系统将用于研究癌症和关节炎的进展，内耳的听力，细胞内的交流，植物的发育和分化，蛋白质合成中的某些RNA催化的反应，蛋白质与细胞膜的相互作用，以及蛋白质的稳定性。最先进的600 MHz核磁共振系统具有14.1Tesla的主动屏蔽磁场，以最大限度地减少其空间需求。它具有四个同时射频激发四种原子核的通道，特别是在生物分子中广泛重要的质子、碳、氮、磷和氢。为了确保光谱干净，伪影少，并能够测量生物分子扩散，该系统包括脉冲场梯度。两个三倍调谐到质子、碳和氮频率的探针将能够表征蛋白质和核酸。一个探头的小体积可以容纳浓缩的样品，而第二个探头的大体积可以容纳只能在更稀溶液中研究的样品。来自四个参与实验室和三个系的五名生物分子核磁共振博士后和五名博士后将使用600兆赫核磁共振光谱仪。在溶液中接受蛋白质结构测定培训的学生目前需求旺盛，这种需求预计将持续多年。600 MHz系统的分辨率更高，灵敏度是校园内500 MHz光谱仪的1.5倍，为更困难的项目提供了便利。这种新的光谱仪位于密苏里大学哥伦比亚分校的校园核磁共振设施中，将帮助结构生物学为生命科学研究的扩展做出贡献，相关领域从医学到农业。