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RAPID: Structure of Membrane-Bound Fusion Peptide of SARS-CoV-2 Required for Infection

RAPID：感染所需的 SARS-CoV-2 膜结合融合肽的结构

基本信息

批准号：
2030473
负责人：
Steven Van Doren
金额：
$ 20万
依托单位：
University of Missouri-Columbia
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2020
资助国家：
美国
起止时间：
2020-06-01 至 2023-05-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2030473&HistoricalAwards=false
关键词：
RAPID Structure Membrane Bound Fusion

项目摘要

The 2019 novel coronavirus spread quickly and became a global pandemic. Infection by an enveloped virus, like SARS-CoV-2, requires fusion of the viral membrane with a host cell membrane. This NSF Rapid Response Research (RAPID) project will support studies to characterize a coronavirus fusion peptide before and after it binds to the cell membrane. The understanding of this process will inform the active development of vaccines and antibodies. The scientific activities will provide a special opportunity for the PI and his lab members to show local high school students how basic research can assist technology development to safeguard public health and safety.The nuclear magnetic resonance (NMR)-guided studies of SARS-CoV-2 docking into the human cells will provide the first structural models of lipid bilayer coordinates bound to a viral fusion peptide, thereby providing details of fusion peptide manipulation of the lipids. The results will prompt immune developers to consider the presence and format of the fusion peptide as antigen. The dynamic structure of the unbound state of the fusion peptide should represent its behavior as a solvent-exposed antigen and the structure of its bilayer-associated state will inform about sites accessible to antibodies outside the bilayer. This RAPID award is made by the Molecular Biophysics Program in the Division of Molecular and Cellular Biosciences, using funds from the Coronavirus Aid, Relief, and Economic Security (CARES) Act.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

2019年新型冠状病毒迅速传播并成为全球大流行病。被包膜病毒（如SARS-CoV-2）感染需要病毒膜与宿主细胞膜融合。NSF快速反应研究（RAPID）项目将支持冠状病毒融合肽在与细胞膜结合之前和之后的特征研究。对这一过程的理解将为疫苗和抗体的积极开发提供信息。科学活动将为PI和他的实验室成员提供一个特殊的机会，向当地高中生展示基础研究如何帮助技术发展，以保障公共健康和安全。核磁共振（NMR）引导的SARS-CoV-2对接到人体细胞的研究将提供第一个与病毒融合肽结合的脂质双层坐标结构模型，从而提供脂质的融合肽操作的细节。结果将促使免疫开发者考虑融合肽作为抗原的存在和形式。融合肽的未结合状态的动态结构应代表其作为溶剂暴露抗原的行为，并且其双层缔合状态的结构将告知双层外抗体可接近的位点。该RAPID奖由分子和细胞生物科学部的分子生物物理计划颁发，使用冠状病毒援助，救济和经济安全（CARES）法案的资金。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。