A Confocal Microscope for the Hopkins Marine Station

摘要汤普森共聚焦激光扫描成像系统将供教师、研究生、本科生和斯坦福大学霍普金斯海洋站的参观者使用。该设备包括蔡司Axiovert SI00倒置显微镜和Noran Oz共聚焦扫描系统。该设备将：i)加强正在进行的教员研究，2)促进新的研究活动，3)促进研究生和博士后对现代光学显微镜方法的培训，以促进他们的研究和职业发展，以及4)加强持续努力，使本科生能够接触到最新的研究技术。四位主要研究人员将使用共聚焦成像系统来解决许多细胞生物学问题，方法范围从离子成像、复杂的荧光显微镜、高分辨率Nomarski DIC到形态计量学。一个研究小组正在研究神经递质激活表面受体后神经元中第二信使信号的空间和时间方面，包括钙离子和一氧化氮信号的动力学。第二个小组正在使用神经解剖学和免疫细胞化学方法研究鱿鱼运动系统的发育和生物物理特性。第三项研究集中于鱼类加热器器官和心脏细胞的特化，使用免疫细胞化学和针对不同亚型的Capumps和肌球蛋白ATPase的抗体。第四组利用荧光成像技术研究了海胆卵受精过程中NO和Ca~(2+)的时空分布规律。主要调查人员正在解决各自学科前沿的问题。这个共享的共焦显微镜中心大大提高了他们进一步发展科学的能力，增强了他们的教学努力，并增加了与斯坦福大学同事和来自其他机构的访客的合作。这与霍普金斯海洋站作为区域海洋生物研究中心的作用是一致的。

Abstract Thompson A confocal laser scanning imaging system will be used by faculty, graduate students, undergraduates and visitors to Stanford University's Hopkins Marine Station. The equipment includes a Zeiss Axiovert SI00 inverted microscope and a Noran Oz confocal scanning system.This equipment will: I) Enhance ongoing faculty research, 2) Promote new research activities, 3) Promote the training of graduate and post-doctoral students in modern methods of optical microscopy in a way that will enhance their research and careerdevelopment, and 4) Strengthen ongoing efforts to permit undergraduatestudents access to the lasted research techniques. The confocal imaging system will be used by four principle investigatorsto address a number of cell biological questions using methods ranging fromion imaging, sophisticated fluorescence microscopy, high resolutionNomarski DIC, to morphometry. One group is investigating the spatial andtemporal aspects of second messenger signaling in neurons following theactivation of surface receptors by neurotransmitters, including thedynamics of Ca2+ and NO signals. A second group is investigating thedevelopment and biophysical properties of motor systems in the squid usingneuroanatomical and immunocytochemical methods. A third study focuses onthe specializations of fish heater organs and cardiac cells usingimmunocytochemistry and antibodies specific for different isoforms of Capumps and myosin ATPase. The fourth group uses fluorescence imagingtechniques to investigate the time course and spatial pattern of NO andCa2+ during fertilization of the sea urchin egg. The principle investigators are addressing questions at the leading edgeof their disciplines. This shared confocal microscopy center addssubstantially to their ability to further their science, enhances theirteaching efforts, and increases collaboration with Stanford colleagues andvisitors from other institutions. This is in keeping with the role of theHopkins Marine Station as a regional center for marine biology research.