POWRE: Development of Biocompatible Sensors Using Endothelial Cells

用于检测生物分子（例如生理流体中的重要离子）的可植入传感器的开发目前已成为越来越多的研究的焦点。常见的可植入传感器的一个主要问题是它们的生物相容性差。身体将这些合成传感器识别为外来物质，并促进蛋白质与表面的不良粘附。这个问题的一个潜在解决方案是将内皮细胞引入传感器表面以增加其生物相容性。内皮细胞排列在血管的内表面，并形成血管壁和流动血液之间的界面。因此，它们对身体不是外来的，并且蛋白质污垢将可能被消除。本项目的目的是通过受体/配体相互作用将人脐静脉内皮细胞（HUVECs）附着到三醋酸纤维素（CTA）膜上，以用作增加生物相容性的离子选择性电极的基础。简言之，CTA膜将用细胞表面受体识别序列Arg-Gly-Asp（RGD）共价固定。然后将内皮细胞接种在改性的CTA膜表面上，并使其在37摄氏度下在5%CO2的潮湿气氛中附着。这项工作将研究的附着和生长的内皮细胞的CTA膜和测试这些表面修饰的膜电极的分析性能。这项工作的最终目标将是优化离子穿过内皮细胞涂层表面膜的运输，从而优化传感器响应，同时保持生物相容性。

0074761AndersonThe development of implantable sensors for detecting biomolecules such as important ions in physiological fluids has currently become the focus of increasing investigation. One major problem with common implantable sensors is their poor biocompatibility. The body recognizes these synthetic sensors as foreign substances and promotes undesirable adhesion of proteins to the surfaces. One potential solution to this problem is to introduce endothelial cells onto the sensor surface to increase its biocompatibility. Endothelial cells line the interior surface of blood vessels and form the interface between the vessel wall and the flowing blood. Consequently, they are not foreign to the body and the protein fouling will possibly be eliminated. The objective of this project is to attach human umbilical veins endothelial cells (HUVECs) to cellulose triacetate (CTA) membranes through receptor/ligand interactions for use as the basis for increased biocompatible ion--selective electrodes. Briefly, the CTA membrane will be covalently immobilized with cell-surface receptor recognition sequence Arg-Gly-Asp (RGD). The endothelial cells will then be seeded on the modified CT A membrane surface and allowed to attach at 37 degrees Celsius in a humidified atmosphere of 5% CO2. The work will investigate the attachment and growth of the endothelial cells to the CTA membranes and test the analytical performance of these surface modified membrane electrodes. The ultimate goal of the work will be to optimize the transport of ions across the endothelial cell coated surface membrane and hence, optimize sensor response while maintaining biocompatibility.