POWRE: Development of Biocompatible Sensors Using Endothelial Cells

POWRE：利用内皮细胞开发生物相容性传感器

• 批准号: 0074761
• 负责人: Kimberly Anderson
• 金额: $ 7.5万
• 依托单位: University of Kentucky Research Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2002-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0074761&HistoricalAwards=false
• 关键词: POWRE; Development; Biocompatible; Sensors; Using

0074761AndersonThe development of implantable sensors for detecting biomolecules such as important ions in physiological fluids has currently become the focus of increasing investigation. One major problem with common implantable sensors is their poor biocompatibility. The body recognizes these synthetic sensors as foreign substances and promotes undesirable adhesion of proteins to the surfaces. One potential solution to this problem is to introduce endothelial cells onto the sensor surface to increase its biocompatibility. Endothelial cells line the interior surface of blood vessels and form the interface between the vessel wall and the flowing blood. Consequently, they are not foreign to the body and the protein fouling will possibly be eliminated. The objective of this project is to attach human umbilical veins endothelial cells (HUVECs) to cellulose triacetate (CTA) membranes through receptor/ligand interactions for use as the basis for increased biocompatible ion--selective electrodes. Briefly, the CTA membrane will be covalently immobilized with cell-surface receptor recognition sequence Arg-Gly-Asp (RGD). The endothelial cells will then be seeded on the modified CT A membrane surface and allowed to attach at 37 degrees Celsius in a humidified atmosphere of 5% CO2. The work will investigate the attachment and growth of the endothelial cells to the CTA membranes and test the analytical performance of these surface modified membrane electrodes. The ultimate goal of the work will be to optimize the transport of ions across the endothelial cell coated surface membrane and hence, optimize sensor response while maintaining biocompatibility.

目前，用于检测生理液体中重要离子等生物分子的植入式传感器的开发已成为人们日益关注的焦点。普通植入式传感器的一个主要问题是它们的生物相容性差。人体将这些合成传感器识别为外来物质，并促进蛋白质在表面的不良粘附。一个潜在的解决方案是将内皮细胞引入传感器表面以增加其生物相容性。内皮细胞排列在血管的内表面，形成血管壁和流动血液之间的界面。因此，他们不是外来的身体和蛋白质污染将可能被消除。该项目的目标是通过受体/配体相互作用将人脐静脉内皮细胞（HUVECs）附着在三醋酸纤维素（CTA）膜上，作为增强生物相容性离子选择电极的基础。简而言之，CTA膜将与细胞表面受体识别序列Arg-Gly-Asp （RGD）共价固定。然后将内皮细胞播种在改良的CT A膜表面，并在37摄氏度的湿度为5%二氧化碳的环境中附着。这项工作将研究内皮细胞在CTA膜上的附着和生长，并测试这些表面修饰膜电极的分析性能。这项工作的最终目标将是优化离子在内皮细胞表面膜上的运输，从而在保持生物相容性的同时优化传感器响应。