Stable isotopes and metabolite profiles as physiological markers for the drought stress sensitivity

本研究的主要目的是从生理生化水平上探讨道格拉斯冷杉种源对干旱的响应。我们的目标是检测种源的干旱敏感性/耐受性的生理和代谢标记。一方面，稳定同位素技术将被应用于在当前的生长季节以及回顾性地在不同的水供应和控制条件下的网站，以确定内在的水分利用效率，气孔反应和蒸腾模式。一个主要的焦点是在碳和氧同位素组成的光合作用后的变化，并将这些因素纳入模型中，以正确地将同位素信号转化为生理参数。代谢物谱的变化将被表征为（i）检测到的干旱胁迫反应的标志物代谢物，并确定（ii）对干旱反应的代谢途径的变化，它们将与器官间碳分配的变化相关。生理反应和代谢标志物的评估在功能上将遗传水平评估（应用程序包的P1）与表型研究（P3）和生态功能评估（P4）联系起来。

The main goal of the research project is to characterise the response of Douglas fir provenances to drought on the physiological and biochemical level. We aim to detect physiological and metabolic markers for drought sensitivity/tolerance of provenances. On the on hand stable Isotope techniques will be applied to characterise intrinsic water use efficiency, stomatal reactions and transpiration patterns during current growing seasons as well as retrospectively at sites varying in water availability and under controlled conditions. One major focus is to characterise post-photosynthetic changes in the carbon and oxygen isotope composition and to include these factors into models to correctly translate isotope signals into physiological parameters. Changes in the metabolite profiles will be characterised to (i) detected marker metabolites for drought stress responses and to identify (ii) changes in metabolic pathways in reaction to drought and they will be linked to changes in inter-organ carbon partitioning. The assessment of physiological reactions and metabolic markers functionally connects the genetic level assessments (P1 of the application package) with phenotypic studies (P3) and assessments of ecological functions (P4).