Stable isotopes and metabolite profiles as physiological markers for the drought stress sensitivity

本研究的主要目的是从生理生化水平上刻画道格拉斯冷杉种源对干旱的响应。我们的目标是检测种源对干旱敏感/耐性的生理和代谢标记。另一方面，稳定同位素技术将被用来描述当前生长季节的内在水分利用效率、气孔反应和蒸腾模式，以及追溯到可用水不同和受控条件下的地点。一个主要的焦点是描述光合作用后碳和氧同位素组成的变化，并将这些因素纳入模型，以正确地将同位素信号转换为生理参数。代谢物图谱的变化将被描述为：(1)检测到干旱应激反应的标记代谢物，并确定(2)对干旱作出反应的代谢途径的变化，这些变化将与器官间碳分配的变化有关。生理反应和代谢标记的评估在功能上将遗传水平评估(应用程序包的P1)与表型研究(P3)和生态功能评估(P4)联系起来。

The main goal of the research project is to characterise the response of Douglas fir provenances to drought on the physiological and biochemical level. We aim to detect physiological and metabolic markers for drought sensitivity/tolerance of provenances. On the on hand stable Isotope techniques will be applied to characterise intrinsic water use efficiency, stomatal reactions and transpiration patterns during current growing seasons as well as retrospectively at sites varying in water availability and under controlled conditions. One major focus is to characterise post-photosynthetic changes in the carbon and oxygen isotope composition and to include these factors into models to correctly translate isotope signals into physiological parameters. Changes in the metabolite profiles will be characterised to (i) detected marker metabolites for drought stress responses and to identify (ii) changes in metabolic pathways in reaction to drought and they will be linked to changes in inter-organ carbon partitioning. The assessment of physiological reactions and metabolic markers functionally connects the genetic level assessments (P1 of the application package) with phenotypic studies (P3) and assessments of ecological functions (P4).