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rRNA Modification Guide RNPs from the Hyperthermophilic Archaeon Pyrococcus Furiosus

rRNA 修饰指南来自超嗜热古细菌 Furiosus 的 RNP

基本信息

批准号：
0077863
负责人：
Michael Terns
金额：
$ 32.88万
依托单位：
University of Georgia Research Foundation Inc
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2000
资助国家：
美国
起止时间：
2000-09-01 至 2004-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0077863&HistoricalAwards=false
关键词：
rRNA Modification Guide RNPs Hyperthermophilic

项目摘要

AbstractMCB-0077863Michael Terns and Rebecca TernsThis project will investigate the structure and function of modification guide ribonucleoproteins (RNPs) in the hyperthermophilic Archaeon Pyrococcus furiosus. A long-term objective of this work is to determine the composition and detailed structural organization of the RNPs and to obtain a mechanistic understanding of how these complexes contribute to rRNA modification and ribosome biogenesis. The proposed work will also provide a foundation for detailed structural questions regarding the nature of the RNP interactions and the molecular basis of thermostability of RNA/protein interactions in hyperthermophilic organisms. The findings should also provide valuable information on the extent of evolutionary conservation of the rRNA processing and modification machinery in Archaea and Eukarya. The specific aims of this project are to identify the protein and RNA components of the modification guide RNPs, to characterize the RNA/protein and protein/protein interactions within the RNPs, and to establish a cell-free rRNA 2'-O-methylation system to study the function of the methylation guide RNPs.AbstractMCB-0077863Michael Terns and Rebecca TernsThis project will investigate the structure and function of modification guide ribonucleoproteins (RNPs) in the hyperthermophilic Archaeon Pyrococcus furiosus. A long-term objective of this work is to determine the composition and detailed structural organization of the RNPs and to obtain a mechanistic understanding of how these complexes contribute to rRNA modification and ribosome biogenesis. The proposed work will also provide a foundation for detailed structural questions regarding the nature of the RNP interactions and the molecular basis of thermostability of RNA/protein interactions in hyperthermophilic organisms. The findings should also provide valuable information on the extent of evolutionary conservation of the rRNA processing and modification machinery in Archaea and Eukarya. The specific aims of this project are to identify the protein and RNA components of the modification guide RNPs, to characterize the RNA/protein and protein/protein interactions within the RNPs, and to establish a cell-free rRNA 2'-O-methylation system to study the function of the methylation guide RNPs.

本项目旨在研究极端嗜热菌Pyrococcus furiosus中修饰引导核糖核蛋白（RNP）的结构和功能。这项工作的一个长期目标是确定RNP的组成和详细的结构组织，并获得这些复合物如何有助于rRNA修饰和核糖体生物合成的机理理解。拟议的工作也将提供一个基础，详细的结构问题的性质的RNP相互作用和分子基础的热稳定性的RNA/蛋白质相互作用在超嗜热生物。这些发现也应该提供有价值的信息的rRNA加工和修饰机制在进化上的保护程度在Eukarya和Eukarya。该项目的具体目标是鉴定修饰指导RNP的蛋白质和RNA组分，表征RNP内的RNA/蛋白质和蛋白质/蛋白质相互作用，建立无细胞rRNA 2 ′-O-甲基化系统，研究甲基化向导RNP的功能. 0077863 Michael Terns和Rebecca Terns本项目将研究极端嗜热菌Pyrococcus furiosus中修饰引导核糖核蛋白（RNP）的结构和功能。这项工作的一个长期目标是确定RNP的组成和详细的结构组织，并获得这些复合物如何有助于rRNA修饰和核糖体生物合成的机理理解。拟议的工作也将提供一个基础，详细的结构问题的性质的RNP相互作用和分子基础的热稳定性的RNA/蛋白质相互作用在超嗜热生物。这些发现也应该提供有价值的信息的rRNA加工和修饰机制在进化上的保护程度在Eukarya和Eukarya。本项目的具体目标是鉴定修饰指导RNP的蛋白质和RNA组分，表征RNP内RNA/蛋白质和蛋白质/蛋白质相互作用，并建立无细胞rRNA 2 '-O-甲基化系统以研究甲基化指导RNP的功能。