Olfaction in Olfactory Bulbectomized Rats

嗅球切除大鼠的嗅觉

• 批准号: 0080746
• 负责人: Burton Slotnick
• 金额: $ 12.28万
• 依托单位: American University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-15 至 2004-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0080746&HistoricalAwards=false
• 关键词: Olfaction; Olfactory; Bulbectomized; Rats

Sensory cells in the nose respond to odors and send signals back to the olfactory bulb of the brain. These cells live for only a few weeks and, when they die, they are replaced by maturing cells from a germinal cell layer in the olfactory tissue. The maturing cells extend their axons into the olfactory bulb where they replace the contacts made by the axons of the dying neuron. The ability of the olfactory receptor epithelium to reconstitute itself in this fashion is unique because neurons in other sensory systems are not replaced if they die. Of particular interest is the fact that if the olfactory bulb is removed, the axons of maturing sensory neurons will continue to grow until they reach brain tissue. They will then make synaptic contact with neurons that do not normally receive inputs from olfactory receptor cells. While this remarkable example of neural plasticity has been demonstrated in anatomicalstudies, it remains unclear whether these new synaptic contacts can be used by the brain to support the sense of smell. We propose to examine this issue by removing the olfactory bulb in neonatal rats and testing their ability to detect and discriminate odors when they are adults. Operated rats will be trained in a precision olfactometer device to determine their odor sensitivity and whether they can discriminate among odors. Behavioral results will be correlated with an anatomical analysis of forebrain connections originating from the olfactory bulb. A positive outcome would provide evidence for a hitherto unrecognized ability of the brain to utilize novel information and translate it into conscious sensory signal

鼻子中的感觉细胞对气味做出反应，并将信号发送回大脑的嗅球。 这些细胞只能存活几周，当它们死亡时，它们会被嗅觉组织中生殖细胞层的成熟细胞所取代。 成熟的细胞将它们的轴突延伸到嗅球，在那里它们取代了垂死神经元的轴突所形成的接触。嗅觉受体上皮以这种方式重建自身的能力是独特的，因为其他感觉系统中的神经元如果死亡则不会被替换。 特别有趣的是，如果嗅球被移除，成熟的感觉神经元的轴突将继续生长，直到到达脑组织。 然后，它们将与通常不接收嗅觉受体细胞输入的神经元进行突触接触。虽然这个神经可塑性的显着例子已经在解剖学研究中得到证实，但仍不清楚这些新的突触接触是否可以被大脑用来支持嗅觉。 我们建议通过切除新生大鼠的嗅球并测试它们成年后检测和辨别气味的能力来研究这个问题。 手术后的老鼠将在精密嗅觉计装置中接受训练，以确定它们的气味敏感性以及它们是否能够区分气味。 行为结果将与源自嗅球的前脑连接的解剖分析相关联。 积极的结果将为大脑利用新信息并将其转化为有意识的感觉信号的能力提供证据。