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Sensing and retrograde signaling of mitochondrial metabolic states in plants

植物线粒体代谢状态的传感和逆行信号传导

基本信息

批准号：
143587254
负责人：
Professorin Dr. Iris Finkemeier
金额：
--
依托单位：
Arbeitsgruppe Plant Physiology
依托单位国家：
德国
项目类别：
Independent Junior Research Groups
财政年份：
2009
资助国家：
德国
起止时间：
2008-12-31 至 2015-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/143587254?language=en
关键词：
Sensing retrograde signaling mitochondrial metabolic

项目摘要

The autotrophic and sessile nature of plants imposes unique demands on their metabolism, and consequently the metabolic network of plants is among the most complex of any organism. To adapt to often rapidily changing environmental conditions the capacity of the metabolic network has to be dynamically adjusted and ‘rewired’ in a way that it is tailored to meet exactly the energy and metabolic demands of the cell at any given moment in time. Plant mitochondria are key players in the cellular metabolism providing the cell with ATP, reducing-power, carbon intermediates and various other important metabolites. The dynamic adjustments of the mitochondrial proteome require signals that sense the status of the organelle and communicate it back to the nucleus. This is referred to as retrograde signaling. In the case of plants, the interpretation of retrograde signals is further complicated by the occurrence of retrograde signals from other organelles such as the plastid. Thus, specificity in signal recognition is a prerequisite. This project aims to investigate the evolutionary conserved process of cell-specific recognition of metabolites in connection with mitochondrial retrograde signaling. Own results demonstrate that perturbations in cellular concentrations of organic acid levels resulting from alterations in tricarboxylic acid cycle activities have a major impact on nuclear gene expression. The aim of this project is to investigate and dissect this mitochondrial retrograde signaling pathway to identify the underlying regulatory mechanisms and components (genes, proteins and metabolites). The approach chosen to tackle this question is based on state of the art genetic and biochemical techniques including expression- and promoter analysis, a forward-genetic screen, the analysis of posttranscriptional modifications and the identification of novel metabolite-binding proteins and signal metabolites.

植物的自养和固着性质对其代谢提出了独特的要求，因此植物的代谢网络是任何生物体中最复杂的。为了适应经常快速变化的环境条件，代谢网络的能力必须动态调整和“重新布线”，以便在任何给定时刻都能满足细胞的能量和代谢需求。植物线粒体是细胞代谢的关键参与者，为细胞提供ATP、还原力、碳中间体和各种其他重要代谢产物。线粒体蛋白质组的动态调整需要能够感知细胞器状态并将其传回细胞核的信号。这被称为逆行信号。在植物的情况下，逆行信号的解释进一步复杂的发生逆行信号从其他细胞器，如质体。因此，信号识别的特异性是先决条件。本项目旨在研究与线粒体逆行信号相关的细胞特异性代谢物识别的进化保守过程。自己的研究结果表明，在细胞浓度的有机酸水平的扰动导致三羧酸循环活动的改变有重大影响的核基因表达。该项目的目的是研究和解剖这种线粒体逆行信号通路，以确定潜在的调控机制和组分（基因，蛋白质和代谢物）。选择解决这个问题的方法是基于最先进的遗传和生化技术，包括表达和启动子分析，正向遗传筛选，转录后修饰的分析和新的代谢物结合蛋白和信号代谢物的鉴定。