Cellular and Molecular Research Techniques in Neuroscience

神经科学中的细胞和分子研究技术

• 批准号: 0089092
• 负责人: Jay Blundon
• 金额: $ 3.22万
• 依托单位: Rhodes College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-01-01 至 2004-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0089092&HistoricalAwards=false
• 关键词: Cellular; Molecular; Research; Techniques; Neuroscience

Biological Sciences (61) As part of the college's plans to enhance the research experience in the natural sciences for our undergraduate students, we are expanding the educational opportunities for students interested in neuroscience. This project is establishing a senior level research course entitled "Molecular and Cellular Research in Neuroscience." For students interested in molecular and cellular neuroscience, this course represents the culminating experience of a new neuroscience major that is to be developed and offered at Rhodes. This new course provides students with more current state-of-the-art research opportunities and experiences, and the new neuroscience major program will stand out as a unique curriculum in the Mid-South region for new undergraduate students of neuroscience. This new research course follows an already successful research-based neuroscience course at Rhodes that employs the more traditional research techniques of cell culture and intracellular and extracellular microelectrode recordings. The new course is based around four fundamental research experiences that have already been developed and published in the neuroscience lab manual "Discovering Neurons: The Experimental Basis of Neuroscience" (Paul et al., 1997). The first laboratory focuses on neurons in development, and involves the measuring of newly expressed ion channel currents in wild type and transfected pheochromo- cytoma (PC12) and COS- 7 cells using whole cell voltage clamp technology. The second laboratory focuses on neurons in communication, and involves the identification of multiple neurotransmitter receptor sites in leech ganglia with dual in situ immunocytochemical labeling techniques. The next laboratory involves neurons forming connections, and incorporates techniques of tract tracing dye injection in the living brain as a method to learn about the afferent and efferent projections in the mammalian cerebrum. The last laboratory is centered on neurons and higher order cognition. In this lab, students research the cellular mechanisms of the synaptic plasticity long- term potentiation by studying synaptic communication in mammalian brain (hippocampal) slices. In this way, students learn how cellular phenomena can result in higher order cognitive process such as learning and memory. Each of the four laboratory experiences offer ample opportunity for further research that can be continued as individually designed independent research experiences.

生物科学（61）作为学院计划的一部分，以提高我们的本科生在自然科学的研究经验，我们正在扩大对神经科学感兴趣的学生的教育机会。该项目正在建立一个高级研究课程，名为“神经科学中的分子和细胞研究”。“对于对分子和细胞神经科学感兴趣的学生来说，这门课程代表了罗兹将开发和提供的一个新的神经科学专业的最终经验。这门新课程为学生提供了更多当前最先进的研究机会和经验，新的神经科学专业课程将成为中南地区神经科学新本科生的独特课程。这个新的研究课程遵循了罗兹已经成功的基于研究的神经科学课程，该课程采用了更传统的细胞培养和细胞内和细胞外微电极记录的研究技术。新课程是基于四个基础研究经验，这些经验已经在神经科学实验室手册《发现神经元：神经科学的实验基础》（Paul et al.，1997年）。第一个实验室专注于发育中的神经元，并涉及使用全细胞电压钳技术测量野生型和转染的嗜铬细胞瘤（PC 12）和COS- 7细胞中新表达的离子通道电流。第二个实验室的重点是神经元的沟通，并涉及多个神经递质受体网站水蛭神经节的双重原位免疫细胞化学标记技术的鉴定。下一个实验室涉及神经元形成连接，并结合了在活体大脑中注射示踪染料的技术，作为了解哺乳动物大脑中传入和传出投射的方法。最后一个实验室集中在神经元和高阶认知。在本实验中，学生借由研究哺乳动物脑（海马）切片中的突触通讯，来研究突触可塑性长时程增强的细胞机制。通过这种方式，学生学习细胞现象如何导致更高层次的认知过程，如学习和记忆。每一个实验室的经验提供了足够的机会，进一步研究，可以继续作为单独设计的独立研究经验。