U. S. Germany Cooperative Research: Phage-type RNA Polymerase(s) and Organellar Transcription in Chlamydomonas

美德合作研究：衣藻噬菌体型RNA聚合酶和细胞器转录

• 批准号: 0089253
• 负责人: David Stern
• 金额: $ 1.44万
• 依托单位: Boyce Thompson Institute Plant Research
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-15 至 2005-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0089253&HistoricalAwards=false
• 关键词: U.; S.; Germany; Cooperative; Research

0089253SternThis award supports David Stern and students from the Boyce Thompson Institute of Cornell University in a collaboration Andreas Weihe of Department of Biology at the Humboldt University in Berlin, Germany. The project will focus on plant organelle ribonucleic acid polymerases (RNAPs) of apparently viral origin. The collaboration brings together unique expertise and facilities: the German laboratory specializes in the requisite molecular tools and the U.S. laboratory in organism-specific techniques. The project was begun by the isolation of an RNAP gene from Chlamydomonas reinhardtii, and initial data indicates that the species might not contain a viral RNAP in its chloroplast, but only in the mitochondria. The specific objective of the research in the next couple of years will be to determine definitively the subcellular localization of the protein encoded by the already cloned nuclear gene, to search for additional homologues, to see conclusively if a viral type RNAP exists in Chlamydomonas chloroplasts, and to investigate transcriptional regulation in mitochondria. Exchange of junior researchers is emphasized to add an international dimension to their training and to allow them to establish international connections early in their careers.

0089253斯特恩该奖项支持大卫斯特恩和学生从博伊斯汤普森研究所康奈尔大学在合作安德烈亚斯Weihe生物系在德国柏林洪堡大学。 该项目将侧重于显然是病毒来源的植物细胞器核糖核酸聚合酶。 这项合作汇集了独特的专业知识和设施：德国实验室专门从事必要的分子工具，美国实验室专门从事生物体特异性技术。 该项目开始于从莱茵衣藻中分离RNAP基因，最初的数据表明该物种可能在其叶绿体中不含病毒RNAP，而仅在线粒体中。 未来几年研究的具体目标将是确定已经克隆的核基因编码的蛋白质的亚细胞定位，寻找其他同源物，最终确定衣原体叶绿体中是否存在病毒型RNAP，并研究线粒体中的转录调控。 强调交流初级研究人员，以增加他们的培训的国际层面，并使他们能够建立国际联系，在他们的职业生涯早期。