Nanopores for Processing Proteins

项目摘要 蛋白质组学领域的最终目标是单分子、高精度、从头蛋白质测序。 不幸的是，目前用于表征和鉴定蛋白质的技术成本高，并且缺乏灵敏度， 动态范围、吞吐量、规模和精度，以满足该领域不断扩大的需求。 在第一阶段SBIR项目期间，Electronic BioSciences，Inc. (EBS)旨在开发一种纳米孔阅读器，沿着 与相关的方法，能够直接，单分子蛋白质测序。一种传感器， 检查单个蛋白质不仅提供关于单个蛋白质本身的详细信息， 它还将使蛋白质组范围的分析和蛋白质表征，生物标志物的发现， 疾病状态进展或缓解的相关评估。这里开发的原型传感器将 是一个高精度的基于纳米孔的阅读器，能够无标记的序列表征，提供高， 高质量数据，无需序列上下文卷积。这一发展将是第一次，非常适合 评估和测序任何给定的蛋白质，而不管长度和结构。此外，灵敏度 所提出的阅读器将能够检测和鉴定特定的翻译后修饰， 对于理解和评估蛋白质组内的广泛变异至关重要。

Project Summary The ultimate goal in the field of proteomics is single-molecule, high-accuracy, de novo protein sequencing. Unfortunately, current technologies used to characterize and identify proteins are costly, and lack the sensitivity, dynamic range, throughput, scale, and accuracy needed to meet the ever-expanding needs within this field. During this Phase I SBIR project, Electronic BioSciences, Inc. (EBS) aims to develop a nanopore reader, along with the associate methodology, capable of direct, single-molecule protein sequencing. A sensor capable of examining single proteins will not only provide detailed information regarding the individual protein themselves, it will also enable proteome-wide profiling and protein characterization, biomarker(s) discovery, and the associated assessments of disease state progression or remission. The prototype sensor developed here will be a high-accuracy nanopore-based reader capable of label-free sequence characterization that provides high- quality data without sequence-context convolution. This development will be the first of its kind, ideally suited to assessing and sequencing any given protein, regardless of length and structure. Additionally, the sensitivity of the proposed reader will enable the ability to detect and identify specific post translational modifications, which is critical to understanding and assessing the extensive variation within the proteome.