An Accurate DNA Base Caller

准确的 DNA 碱基识别器

• 批准号: 0090738
• 负责人: Mohamad Musavi
• 金额: $ 42.5万
• 依托单位: University of Maine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2004-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0090738&HistoricalAwards=false
• 关键词: Accurate; DNA; Base; Caller

The major goal of this project is to develop a new base calling technique that will improve the efficiency of the DNA sequencing process. This will be achieved by increasing the average length of error-free sequencing and enhancing the base identification process at the beginning and end of sequences. This will increase sequencing throughput and reduce the cost of DNA sequencing. Previous work by the PI has demonstrated the ability to extend the error-free read by 30%. This was achieved through work on cross-talk filtering, baseline adjustment, base-spacing prediction and development of a fuzzy base-calling algorithm. Further adaptive capabilities as well as full development and implementation of the methodology is planned. The software will be tested on a large number of DNA sequences and remove specific hardware and operating system requirements, as well as be exploitable over the web. Accurate, inexpensive genomic DNA sequencing will be a cornerstone of 21st century biology.

该项目的主要目标是开发一种新的碱基调用技术，以提高DNA测序过程的效率。这将通过增加无错误测序的平均长度和加强序列开始和结束的碱基识别过程来实现。这将提高测序通量，降低DNA测序成本。PI之前的工作已经证明了将无错误读取扩展30%的能力。这是通过串扰滤波、基线调整、基间距预测和模糊基调用算法的开发来实现的。进一步的适应能力以及全面开发和实施该方法的计划。该软件将在大量DNA序列上进行测试，去除特定的硬件和操作系统要求，并可通过网络进行开发。准确、廉价的基因组DNA测序将成为21世纪生物学的基石。