CAREER: Aqueous Two-Phase Separation of DNA Using PNA-Conjugated Amphiphiles
事业:使用 PNA 共轭两亲物对 DNA 进行水相两相分离
基本信息
- 批准号:0093538
- 负责人:
- 金额:$ 37.5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2001
- 资助国家:美国
- 起止时间:2001-02-01 至 2007-01-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The PI proposes experiments to identify ATPS polymers and operating conditions so that tagged DNA will strongly partition to one phase, leaving polar proteins and untagged DNA in the other phase. By proper molecular design, the PNA amphiphile will dissociate from the DNA product upon heating the system above the melting transition temperature (Tm) of the PNA-DNA recognition complex. He will also describe a series of theoretical and experimental techniques that will be used to design PNA amphiphiles so that they specifically bind plasmid DNA, but have Tm in a useful range for ATPS. In addition to the development of a useful biochemical process, molecular-level design of the PNA amphiphile-DNA binding complex proposed here should also shed light on the contribution of intermolecular forces to the structure and function of biomolecular materials.His education plan aims to cultivate molecular-level insight in the chemical engineering curriculum by a series of short modules to be assigned in core chemical engineering courses. Molecular modeling and experimental results of the research proposed here will be integrated into the modules, which are designed to predict fundamental engineering properties such as thermal conductivity, viscosity, and vapor-liquid equilibrium from molecular parameters. Another educational effort he proposes is a two-week mini-course for a colloid/interfacial engineering course that emphasizes the connection between biotechnology and colloid science.
PI提出实验来鉴定ATPS聚合物和操作条件,使得标记的DNA将强烈地分配到一个相,将极性蛋白质和未标记的DNA留在另一相中。通过适当的分子设计,PNA两亲物将在将系统加热到高于PNA-DNA识别复合物的解链转变温度(Tm)时从DNA产物解离。他还将描述一系列理论和实验技术,将用于设计PNA两亲物,使它们特异性结合质粒DNA,但具有在ATPS的有用范围内的Tm。除了开发有用的生物化学过程外,分子水平设计的PNA两亲物-DNA结合复合物还可以阐明分子间力对生物分子材料结构和功能的贡献。他的教育计划旨在通过化学工程核心课程中的一系列短模块来培养化学工程课程中的分子水平洞察力。这里提出的研究的分子建模和实验结果将被集成到模块中,这些模块旨在从分子参数预测基本的工程性质,如热导率,粘度和汽液平衡。他提出的另一项教育努力是为胶体/界面工程课程开设一个为期两周的迷你课程,强调生物技术和胶体科学之间的联系。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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James Schneider其他文献
152 Trade-Off of Different Auto-Segmentation Approaches in the Case of Pediatric Craniospinal Irradiation
152 不同自动分割方法在小儿颅脊髓照射情况下的权衡
- DOI:
10.1016/s0167-8140(24)03642-9 - 发表时间:
2024-09-01 - 期刊:
- 影响因子:5.300
- 作者:
Alana Thibodeau-Antonacci;Ozgur Ates;Chia-ho Hua;Marija Popovic;James Schneider;Sonia Skamene;Carolyn Freeman;Shirin Enger;James M.G. Tsui - 通讯作者:
James M.G. Tsui
Defining leadership competencies for pediatric critical care fellows: Results of a national needs assessment
定义儿科重症监护人员的领导能力:国家需求评估的结果
- DOI:
- 发表时间:
2017 - 期刊:
- 影响因子:4.7
- 作者:
Michael L. Green;M. Winkler;R. Mink;Melissa L. Brannen;M. Bone;T. Maa;G. Arteaga;M. Mccabe;Karen J. Marcdante;James Schneider;D. Turner - 通讯作者:
D. Turner
ACUTE KIDNEY INJURY, AS DEFINED BY THE RIFLE CRITERIA, IS ASSOCIATED WITH MORTALITY AND LENGTH OF STAY IN CHILDREN UNDERGOING SURGERY FOR CONGENITAL HEART DISEASE
- DOI:
10.1016/s0735-1097(11)60450-6 - 发表时间:
2011-04-05 - 期刊:
- 影响因子:
- 作者:
Scott Aydin;Howard Seiden;Andrew Blaufox;Vincent Parnell;Tarif Choudhury;Ann Punnoose;James Schneider - 通讯作者:
James Schneider
The Global Technology Revolution
全球技术革命
- DOI:
- 发表时间:
2001 - 期刊:
- 影响因子:0
- 作者:
P. Antón;R. Silberglitt;James Schneider - 通讯作者:
James Schneider
Best Practices in Medical Documentation: A Curricular Module
- DOI:
10.1016/j.acap.2022.02.006 - 发表时间:
2022-11-01 - 期刊:
- 影响因子:
- 作者:
Megan E. McCabe;Richard Mink;David A. Turner;Donald L. Boyer;Mohammad Hossein Tcharmtchi;Jason Werner;James Schneider;Veronica Armijo-Garcia;Margaret Winkler;David Baker;Katherine E. Mason - 通讯作者:
Katherine E. Mason
James Schneider的其他文献
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{{ truncateString('James Schneider', 18)}}的其他基金
Rapid, Free-Solution Electrophoretic Separations of Kilobase DNA by Transiently Attached Wormlike Micelles
通过瞬时附着的蠕虫状胶束对千碱基 DNA 进行快速、自由溶液电泳分离
- 批准号:
1605351 - 财政年份:2016
- 资助金额:
$ 37.5万 - 项目类别:
Standard Grant
Symposium: Participant Travel Support for the 89th ACS Colloid and Surface Science Symposium, June 15-17, 2015, Pittsburgh, PA
研讨会:第 89 届 ACS 胶体和表面科学研讨会的与会者旅行支持,2015 年 6 月 15 日至 17 日,宾夕法尼亚州匹兹堡
- 批准号:
1523306 - 财政年份:2015
- 资助金额:
$ 37.5万 - 项目类别:
Standard Grant
UNS: Mechanisms of Surface Charging in Nonpolar Media
UNS:非极性介质中的表面充电机制
- 批准号:
1511619 - 财政年份:2015
- 资助金额:
$ 37.5万 - 项目类别:
Standard Grant
Rapid Electrophoretic Sorting of DNA using Nanoemulsion Tags
使用纳米乳液标签对 DNA 进行快速电泳分选
- 批准号:
0932536 - 财政年份:2009
- 资助金额:
$ 37.5万 - 项目类别:
Standard Grant
MRI: Development of Fluorescence-Based Spectroscopy and Imaging Microfluidics System for Surface Chemical and Geometric Optimization
MRI:开发基于荧光的光谱和成像微流体系统,用于表面化学和几何优化
- 批准号:
0320548 - 财政年份:2003
- 资助金额:
$ 37.5万 - 项目类别:
Standard Grant
NER: Chemical Probing of Biosensor Nano-environments using Dynamic AFM
NER:使用动态 AFM 对生物传感器纳米环境进行化学探测
- 批准号:
0210205 - 财政年份:2002
- 资助金额:
$ 37.5万 - 项目类别:
Standard Grant
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