MRI: Development of Fluorescence-Based Spectroscopy and Imaging Microfluidics System for Surface Chemical and Geometric Optimization

MRI：开发基于荧光的光谱和成像微流体系统，用于表面化学和几何优化

• 批准号: 0320548
• 负责人: James Schneider
• 金额: $ 10.64万
• 依托单位: Carnegie-Mellon University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2004-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0320548&HistoricalAwards=false
• 关键词: MRI; Development; Fluorescence; Based; Spectroscopy

ABSTRACTProposal No. CTS-0320548Principal Investigator: J. Schneider, Carnegie Mellon UniversityThis grant is to develop a fluorescence-based instrument for the visualization of flow and adsorption in microfluidic systems. The instrument consists of an inverted epifluorescence microscope, a solid-state laser, a high-speed CCD camera, a low-noise DC power supply, and some smaller equipment. Measurement of adsorption will be accomplished by total internal reflection fluorescence (TIRF). Microfluidic devices will be constructed using standard soft lithography methods, allowing for control of microchannel geometry and surface chemistry. Fluid flow will be induced either by electroosmosis or a syringe pump, depending on the application.Three research projects, representative of those to be supported by this equipment, are as follows: The first is an assessment of the contribution of lateral diffusion to the kinetics of DNA hybridization to surface-bound probes as means to improve the performance of biosensors. The second is the development of a microscale chromatography system that utilizes DNA-binding surfactants to isolate target DNA from complex mixtures. The third is the development and validation of numerical algorithms for improved computer-aided design of lab-on-a-chip systems.The impact of the instrumentation will be on many fronts. This is perhaps the first microfluidic visualization instrument that includes on-line TIRF, supplying surface adsorption information critical to the above research. In addition, the many groups on campus involved with microfluidics and MEMS would have this instrument as a shared resource. We will also perform a series of microfluidics experiments (available as a web-based resource) to serve as examples and problem sets for transport courses. We will also develop hands on projects for high school students participating in the Summer Academy for Minority Students at Carnegie Mellon University. This would serve as an introduction to an exciting field and familiarize students with state-of-the-art lab equipment.

摘要建议编号 CTS-0320548主要研究者： J. Schneider，Carnegie Mellon University该基金将开发一种基于荧光的仪器，用于微流体系统中流动和吸附的可视化。该仪器由倒置荧光显微镜、固体激光器、高速CCD摄像机、低噪声直流电源和一些小型设备组成。吸附的测量将通过全内反射荧光（TIRF）完成。 微流控器件将使用标准的软光刻方法构建，允许控制微通道几何形状和表面化学。流体流动将诱导通过电渗或注射泵，这取决于application.Three研究项目，代表那些将由该设备支持，如下：第一个是横向扩散的DNA杂交的动力学的贡献的评估表面结合的探针作为手段，以提高生物传感器的性能。第二个是开发一种微型色谱系统，利用DNA结合表面活性剂从复杂的混合物中分离目标DNA。第三是开发和验证用于改进芯片实验室系统的计算机辅助设计的数值算法。这可能是第一个包括在线TIRF的微流体可视化仪器，为上述研究提供关键的表面吸附信息。 此外，校园里许多涉及微流体和MEMS的团体将把这种仪器作为共享资源。 我们还将进行一系列的微流体实验（可作为基于网络的资源），作为例子和运输课程的问题集。我们还将为参加卡内基梅隆大学少数民族学生暑期学院的高中生开发动手项目。 这将作为一个令人兴奋的领域的介绍，并使学生熟悉最先进的实验室设备。