U.S.-Hungary Research on High Density Mapping of Genes Responsible for Frost Tolerance in Wheat

美国-匈牙利对小麦抗霜冻基因高密度作图研究

• 批准号: 0095051
• 负责人: Jorge Dubcovsky
• 金额: $ 3.88万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0095051&HistoricalAwards=false
• 关键词: U.S.; Hungary; Research; Density; Mapping

INT 0095059DubcovskyThis U.S.-Hungarian research involves Jorge Dubcovsky of the University of California, Davis, and Gabor Galiba of the Hungarian Agricultural Research Institute, Martonvasar, and features the isolation and characterization of genes affecting frost tolerance in wheat. The researchers will combine the genetic models and tools for diploid wheat, Triticum monococcum, developed at Davis with the characterization procedures for frost tolerance and vernalization developed at Martonvasar. Their goal is to determine how to cone the genes responsible for frost tolerance in wheat.Using a combination of positional cloning and differential display approaches, their joint research plan includes: 1) construction of a detailed chromosomal map for a new found gene affecting frost tolerance in wheat, Fr-Am2, on the chromosome 5AmL of T. monococcum; 2) mapping the regulatory loci of winter hardiness, cor14b, a cold-inducible expression on T. monococcum; and 3) mapping the stress induced genes, identified by differential display and deletion and substitution of chromosome 5A in hexaploid wheat. Successfully identified cDNA clones will be incorporated into T. monococcum maps.Molecular markers tightly linked to the frost tolerance genes will be used to screen the Bacterial Artificial Chromosome (BAC) library of T. monococcum to characterize the complete genes and their regulatory elements. Results are expected to relate controlling loci to inducible genes. If successful, the new information may contribute to our basic understanding of the physiological and biochemical bases for freeze tolerance, which is an agriculturally important plant trait. This international project in plant genome research fulfills the program objective of advancing scientific knowledge by enabling experts in the Untied States and Central Europe to combine complementary talents and share research resources in areas of strong mutual interest and competence.

Int 0095059 Dubcovsky这项美国-匈牙利的研究涉及加州大学戴维斯分校的Jorge Dubcovsky和匈牙利农业研究所Martonvarar的Gabor Galiba，其特点是分离和表征了影响小麦耐霜性的基因。研究人员将把戴维斯开发的二倍体小麦--单粒小麦--的遗传模型和工具与马尔顿瓦萨开发的耐霜性和春化鉴定程序结合起来。利用定位克隆和差异显示相结合的方法，他们的联合研究计划包括：1)构建新发现的影响小麦耐霜性的基因Fr-Am2的详细染色体图谱；2)定位小麦抗寒性的调控基因cor14b；3)通过差异显示和六倍体小麦5A染色体的缺失和替换鉴定胁迫诱导基因。利用与抗霜性基因紧密连锁的分子标记筛选单球菌细菌人工染色体(BAC)文库，以确定完整的基因及其调控元件。预计结果将把控制基因与诱导基因联系起来。如果成功，这些新的信息可能有助于我们基本了解耐寒性的生理和生化基础，耐冻性是一种重要的农业植物性状。这一植物基因组研究的国际项目通过使美国和中欧的专家能够结合互补的人才并在相互感兴趣和能力很强的领域共享研究资源，实现了促进科学知识的计划目标。