Biological function of aureochromes in the diatom Phaeodactylum tricornutum

硅藻三角褐指藻金色素的生物学功能

• 批准号: 146946742
• 负责人: Professor Dr. Peter Kroth
• 金额: --
• 依托单位: Arbeitsgruppe Pflanzenphysiologie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/146946742?language=en
• 关键词: Biological; function; aureochromes; diatom; Phaeodactylum

Diatoms are unicellular algae that can be found in any aquatic habitat. Being mainly photoautotrophs, diatoms use light as a driving force for photosynthesis but also for cellular regulation. Wehave shown that a blue light receptor is responsible for triggering the metabolic changes which induce high light acclimation. There is some evidence that aureochromes (AUREOs) which are blue light receptors and transcriptions factors possessing a bZIP and a LOV domain may be responsible for this light acclimation reaction. We have generated Aureo mutants which are significantly decreased in AUREO1a on the protein level. These mutants will be studied on the physiological level if the light acclimation reaction is impaired. On the genomic level we want to identify cis-acting elements as well as interaction partners of the AUREO proteins.

硅藻是单细胞藻类，可以在任何水生栖息地找到。作为主要的光合自养生物，硅藻利用光作为光合作用的驱动力，但也用于细胞调节。我们已经表明，蓝光受体是负责触发代谢的变化，诱导高光驯化。有一些证据表明，具有bZIP和LOV结构域的蓝光受体和转录因子金色素（AUREO）可能负责这种光适应反应。我们已经产生了Aureo突变体，其在蛋白质水平上显著降低AureO1a。这些突变体将在生理水平上进行研究，如果光适应反应受损。在基因组水平上，我们希望确定顺式作用元件以及AUREO蛋白的相互作用伙伴。