Laboratory Equipment for Post-Genomic Studies in Environmental Biology

环境生物学后基因组研究的实验室设备

• 批准号: 0100193
• 负责人: Mitchell Sogin
• 金额: $ 19.89万
• 依托单位: Marine Biological Laboratory
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-06-15 至 2003-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0100193&HistoricalAwards=false
• 关键词: Laboratory; Equipment; Post; Genomic; Studies

This award provides support for purchase of equipment to establish an integrated laboratory for high-throughput processing of DNA samples in environmental research. Establishment of the laboratory will give scientists at the Marine Biological Laboratory (MBL) and the Woods Hole Oceanographic Institution (WHOI) access to high-throughput capabilities that are typically restricted to biomedical or industrialized genomic research centers. Equipment to be purchased include several robotic workstations, a real-time quantitative polymerase chain reaction device and a pulsed field gel electrophoresis) system. This new equipment will permit scientists at both institutions to achieve optimum use of an existing high capacity DNA sequencing and microarray facility. This robotic equipment will completely automate management and processing of DNA samples, and thus enable scientists to sample genetic diversity in the environment at a level previously unimaginable. Research questions to be pursued address issues in genome evolution, biodiversity, and environmental influences on gene expression. The equipment will allow precise measurement of changes in gene expression in response to external factors (e.g. toxins, other chemicals) and as a result of symbiosis, and will also be used to measure specific DNA sequences in uncultivable environmental populations. The new high-throughput capabilities will also directly impact the training and education of visiting and year-round scientists, students, and postdoctoral fellows at the two institutions in Woods Hole, MA.

这笔拨款为购买设备提供支持，以建立一个综合实验室，在环境研究中对DNA样本进行高通量处理。该实验室的建立将使海洋生物实验室（MBL）和伍兹霍尔海洋研究所（WHOI）的科学家获得通常仅限于生物医学或工业化基因组研究中心的高通量能力。购买的设备包括几个机器人工作站，一个实时定量聚合酶链反应装置和一个脉冲场凝胶电泳系统。这种新设备将使两家机构的科学家能够最大限度地利用现有的高容量DNA测序和微阵列设备。这种机器人设备将完全自动化DNA样本的管理和处理，从而使科学家能够以以前难以想象的水平对环境中的遗传多样性进行采样。研究问题要追求解决的问题在基因组进化，生物多样性和环境对基因表达的影响。该设备将允许精确测量外部因素（例如毒素，其他化学物质）和共生导致的基因表达变化，也将用于测量不可培养环境种群中的特定DNA序列。新的高通量能力也将直接影响马萨诸塞州伍兹霍尔两所机构的访问和全年科学家、学生和博士后研究员的培训和教育。