Microbial Genome Sequencing: Microsporidia and the Next Generation of Genome Scientists

微生物基因组测序：小孢子虫和下一代基因组科学家

• 批准号: 0135272
• 负责人: Mitchell Sogin
• 金额: --
• 依托单位: Marine Biological Laboratory
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-10-01 至 2007-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0135272&HistoricalAwards=false
• 关键词: Microbial; Genome; Sequencing; Microsporidia; Next

Except for a small number of human pathogens, microbial genome efforts have steered clear of the diverse world of protozoans, many of which are important agricultural parasites. Of particular interest are the Microsporidia, which by eukaryotic standards have remarkably small genomes. Since they infect most invertebrate taxa and all classes of vertebrates, their impact on agriculture and fisheries is significant. They either cause disease or can serve as bio-control agents. These same organisms have attracted the interests of biologists and molecular evolutionists. They offer excellent models for studying interactions between the genomes of a host and an obligate, intracellular parasite. But molecular phylogenetic analyses point towards a confused evolutionary history. To advance knowledge of these unusual microbes and to address the ever-increasing demand for researchers broadly trained in genome sciences, the genome of the microsporidian Nosema locustae will be sequenced to closure and a comprehensive course in genome science will be established to provide broad-based training in high throughput sequencing techniques and bioinformatics. By integrating this course with the N. locustae project, nearly 100 students will be trained in all aspects of genomics. Although this model for research and training could be applied to other microbial groups, Microsporidia were selected because of their impact upon agriculture, their enigmatic character in molecular evolution studies and what they may reveal about interactions between the genomes of hosts and obligate intracellular parasites. Sequence data (3X and higher coverage, monthly updates and annotation) will be available to the research community on the Marine Biological Laboratory's web server (www.mbl.edu). This is a Microbial Genome Sequencing Award funded through a collaborative program between the National Science Foundation and the Department of Agriculture Initiative for Future Agriculture and Food Systems

除了少数人类病原体，微生物基因组研究已经避开了原生动物的多样性世界，其中许多是重要的农业寄生虫。特别令人感兴趣的是微孢子虫，按照真核生物的标准，它的基因组非常小。由于它们感染大多数无脊椎动物分类群和所有脊椎动物，因此对农业和渔业的影响很大。它们要么引起疾病，要么可以作为生物控制剂。这些有机体吸引了生物学家和分子进化论者的兴趣。它们为研究宿主基因组与专性细胞内寄生虫之间的相互作用提供了极好的模型。但是分子系统发育分析指出了一个混乱的进化历史。为了提高对这些不寻常的微生物的认识，并满足对在基因组科学方面受过广泛培训的研究人员日益增长的需求，将对蝗虫微孢子虫的基因组进行测序，并将建立一个全面的基因组科学课程，以提供高通量测序技术和生物信息学方面的广泛培训。通过将本课程与N.在蝗虫项目中，近100名学生将接受基因组学各个方面的培训。虽然这种研究和训练模型可以应用于其他微生物类群，但选择微孢子虫是因为它们对农业的影响，它们在分子进化研究中的神秘特性以及它们可能揭示宿主基因组与专性细胞内寄生虫之间的相互作用。序列数据（3X和更高覆盖率，每月更新和注释）将在海洋生物实验室的网络服务器（www.mbl.edu）上提供给研究界。这是一个微生物基因组测序奖，由美国国家科学基金会和农业部未来农业和食品系统倡议之间的合作计划资助。