A Real-Time Sequence Detection System for the Center for Marine Genomic Studies

该合同支持购买沙漠山岛生物实验室海洋基因组研究中心进行基因组DNA分析所需的仪器。将购买的两台仪器是第二代聚合酶链式反应(PCR)热循环仪，带有四通道荧光微孔板读取器，可以监测过程中的反应，以及机器人微孔板处理器，可以有效地设置聚合酶链式反应设备中使用的96孔板。这些仪器将促进对海洋生物中基因的表达和功能的研究，特别强调增进对与盐度和温度等环境决定因素有关的生理过程的了解。四个由美国国家科学基金会资助的研究项目将主要使用所要求的仪器。这些研究集中在对真盐目硬骨鱼和甲壳类鱼类鳃中膜运输蛋白和相关酶的分子研究。

This award supports the purchase of instrumentation needed for genomic DNA analysis at the Center for Marine Genomic Studies of the Mount Desert Island Biological Laboratory. The two instruments to be purchased are a second-generation polymerase chain reaction (PCR) thermal cycler with four-channel fluorescence microplate reader that permits monitoring of reactions in process, and a robotic microplate processor that allows efficient set up of the 96-well plates used in the PCR device. The instruments will facilitate studies of the expression and function of genes in marine organisms with particular emphasis on improving the understanding of physiological processes in relation to environmental determinants such as salinity and temperature. Four NSF-funded research projects will make major use of the requested instruments. These focus on molecular studies of membrane transport proteins and associated enzymes in the gills of euryhaline teleost fish and of crustaceans.