Molecular and Physiological Characterization of Anaerobic Microbial Communities that Reductively Dechlorinate Chlorinated Ethenes

氯化乙烯还原脱氯的厌氧微生物群落的分子和生理学特征

• 批准号: 0104740
• 负责人: Lisa Alvarez-Cohen
• 金额: $ 50.34万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至 2005-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0104740&HistoricalAwards=false
• 关键词: Molecular; Physiological; Characterization; Anaerobic; Microbial

0104740 Alvarez-Cohen The objectives of this research are to characterize the microbial diversity in consortia capable of reductively dechlorinating chlorinated ethenes to determine the roles of different physiological groups in those reactions, to isolate pure cultures capable of complete dechlorination of trichloroethene (TCE), and to construct simple consortia capable of rapid, stable growth and efficient, complete dechlorination of TCE. Various molecular techniques will be used in this study, including clone library construction/analysis, terminal restriction fragment length polymorphism (T-RFLP) analysis, and fluorescence in-situ hybridization (FISH) analysis. The anticipated results of this study will provide fundamental information on the diversity, physiology and biochemistry of microbial species involved in complete reductive dechlorination of chlorinated ethenes. This information is needed for designing efficient treatment processes for remediating chlorinated ethene contaminated sites. This research project will advance the development of molecular biological techniques as tools for evaluating and monitoring reductive dechlorination in contaminated subsurface environments. Also, it could lead to the development of microbial consortia appropriate for application in bioaugmentation strategies for site remediation. ***

0104740 Alvarez-Cohen本研究的目的是表征能够还原脱氯氯化乙烯的财团中的微生物多样性，以确定这些反应中不同生理基团的作用，分离能够完全脱氯三氯乙烯（TCE）的纯培养物，并构建能够快速，稳定生长和有效，完全脱氯三氯乙烯的简单财团。 本研究将使用各种分子技术，包括克隆文库构建/分析、末端限制性片段长度多态性（T-RFLP）分析和荧光原位杂交（FISH）分析。 本研究的预期结果将提供基本信息的多样性，生理和生物化学的微生物物种参与完全还原脱氯的氯化乙烯。 这一信息是设计有效的处理过程，补救氯化乙烯污染的网站所需要的。 这一研究项目将推动分子生物学技术的发展，作为评估和监测受污染地下环境中还原脱氯的工具。 此外，它可能导致微生物财团的发展，适用于现场修复的生物强化策略。***