Quantifying Gene Expression to Predict and Optimize Reductive Dechlorination by Dehalococcoiides spp.

量化基因表达以预测和优化 Dehalococcoiides spp 的还原脱氯。

• 批准号: 0504244
• 负责人: Lisa Alvarez-Cohen
• 金额: --
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0504244&HistoricalAwards=false
• 关键词: Quantifying; Gene; Expression; Predict; Optimize

0504244 Alvarez-Cohen The proposed work would provide more insight into the role of multiple Dehalococcoides strains in dechlorination of chlorinated solvents by comparing measured 16S and expressed genes for known reductases. There would also be potential discovery of novel reductases. The plan also calls for study of a range of community structure from pure cultures of strain 195 to combinations of known cultures to well characterized mixed cultures to mixed cultures that have had relatively little characterization. These could reveal identities of synergistic species important to the ecology of Dehalococcoides populations. However, the latter result is probably the least likely. The intellectual merit of the project is strong and the broader impacts clearly articulated.

0504244 Alvarez-Cohen拟议的工作将通过比较已知还原酶的测量的16 S和表达的基因，对多种Dehalococcoides菌株在氯化溶剂脱氯中的作用提供更多的见解。也有可能发现新的还原酶。该计划还要求研究一系列的社区结构，从菌株195的纯培养物到已知培养物的组合，再到特征良好的混合培养物，再到特征相对较少的混合培养物。这些可以揭示身份的协同物种重要的生态Dehalococcoides人口。然而，后一种结果可能性最小。该项目的知识价值很强，更广泛的影响得到了明确阐述。