SBIR Phase I: Microfluidic Protein Crystallization Device with Valves

SBIR 第一阶段：带阀门的微流控蛋白质结晶装置

• 批准号: 0109845
• 负责人: Robert Haushalter
• 金额: $ 9.94万
• 依托单位: Parallel Synthesis Technologies, Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2001-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0109845&HistoricalAwards=false
• 关键词: SBIR; Phase; Microfluidic; Protein; Crystallization

0109845HaushalterThis Small Business Innovation Research Phase I project will develop micromachined fluid handling components as part of a new technology platform for high throughput protein crystallization and the collection of single crystal x-ray diffraction data. As increasing effort is directed toward proteomics and high throughput proteinstructure determination, the data derived from the determination of 3-D protein structureswill have enormous impact in such areas as protein engineering, recombinant DNA technology and gene therapy. This project proffers a simple design for an inexpensive device, a Microfluidic Protein Crystallization Chip (MPCC), that possesses a high-density array of vessels, each with fluid inlets and vents, and a design for simple on-chip two-way valves. Methods are proposed to rapidly prototype new designs with design modifications made on the order of a day. The MPCC will be able to dose solutions appropriate to run commercial screening matrices. Since the device will be on the order of 1 mm thick, x-ray data will be collected by simply placing the crystal-containing MPCC device directly into the x-ray beam, thereby abrogating the expensive labor and equipment intensive exercise of isolating several micron sized crystals from 20-100 nL of mother liquid.The commercial applications of this project will be in the Structural Genomics market.

0109845 Haushalter这个小型企业创新研究第一阶段项目将开发微机械流体处理组件，作为高通量蛋白质结晶和单晶X射线衍射数据收集的新技术平台的一部分。随着人们对蛋白质组学和高通量蛋白质结构测定研究的不断深入，蛋白质三维结构测定数据将在蛋白质工程、重组DNA技术和基因治疗等领域产生巨大的影响。该项目提供了一个简单的设计，一个廉价的设备，微流体蛋白质结晶芯片（MPCC），拥有一个高密度的容器阵列，每个容器都有流体入口和出口，以及一个简单的芯片上的双向阀的设计。提出了方法，以快速原型新的设计与设计修改的订单上的一天。MPCC将能够投配适合于运行商业筛选基质的溶液。由于该装置的厚度约为1 mm，因此通过简单地将含有晶体的MPCC装置直接放置到X射线束中来收集X射线数据，从而消除了从20-100 nL母液中分离几个微米大小的晶体的昂贵的劳动力和设备密集型操作。