SGER: Multicolor Total Internal Reflection Fluorescence Microscopy

SGER：多色全内反射荧光显微镜

• 批准号: 0110070
• 负责人: Sanford Simon
• 金额: $ 9.94万
• 依托单位: Rockefeller University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0110070&HistoricalAwards=false
• 关键词: SGER; Multicolor; Total; Internal; Reflection

0110070SimonThe primary goal of this proposal is to develop techniques to image two colors simultaneously at video rates (30 frames/sec) so that either two different probes, or a single probe with two different emissions, can be imaged synchronously. Further, to develop the techniques for using multiple lasers for excitation. This goal will allow two different kinds of studies: First, it would allow multiple proteins to be studied in a single cell. This is important for addressing questions of protein-protein interactions. Second, it will allow many physiological studies using dual-emission dyes that can be used to quantify important cell signaling molecules such as calcium or pH. The spatial and temporal dynamics of these molecules are key for understanding many aspects of cellular signaling.

0110070西蒙该提案的主要目标是开发以视频速率（30帧/秒）同时成像两种颜色的技术，使得两个不同的探头或具有两种不同发射的单个探头可以同步成像。此外，开发用于使用多个激光器进行激发的技术。这一目标将允许两种不同类型的研究：首先，它将允许在单个细胞中研究多种蛋白质。这对于解决蛋白质-蛋白质相互作用的问题很重要。其次，它将允许许多生理学研究使用双发射染料，可用于量化重要的细胞信号分子，如钙或pH值。这些分子的空间和时间动态是理解细胞信号的许多方面的关键。