Biophotonics: In situ Optical Imaging of Multiple Proteins

生物光子学：多种蛋白质的原位光学成像

• 批准号: 0119468
• 负责人: Sanford Simon
• 金额: $ 61.17万
• 依托单位: Rockefeller University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-11-15 至 2004-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0119468&HistoricalAwards=false
• 关键词: Biophotonics; situ; Optical; Imaging; Multiple

0119468SimonThe objective of the proposed research is to develop a method of optically imaging proteins. This research will adapt newly developed methods for peptide/protein ligation for labeling various proteins and imaging them via improved fluorescence or other molecular imaging probes. The ability to study proteins in situ has come from the introduction of the green Fluorescent protein (GFP). The goal of this research is to generate reporters that share the power of GFP, but without its limitations. The specific goals of this research are to generate reporters that: (1) can be genetically engineered into proteins, (2) are significantly smaller than GFP, (3) have a narrower emission spectra than GFP, (4) emit further in the red range or have a larger Stokes shift than GFP, (5) are more photostable than GFP, (6) can be used to simultaneously label and distinguish different proteins in living cells, and (7) can be used to track proteins or cells as they move throughout an animal's body.

0119468西蒙提出的研究的目的是开发一种光学成像蛋白质的方法。 这项研究将采用新开发的肽/蛋白质连接方法来标记各种蛋白质，并通过改进的荧光或其他分子成像探针对其进行成像。 原位研究蛋白质的能力来自于绿色荧光蛋白（GFP）的引入。 这项研究的目标是产生报告人，分享GFP的力量，但没有其限制。 这项研究的具体目标是产生报告人：（1）可以被遗传工程改造成蛋白质，（2）比GFP显著小，（3）具有比GFP更窄的发射光谱，（4）在红色范围内发射得更远或具有比GFP更大的斯托克斯位移，（5）比GFP更耐光，（6）可用于同时标记和区分活细胞中的不同蛋白质，以及（7）可用于跟踪蛋白质或细胞在动物体内的移动。