Multicolor Cell sorter

多色细胞分选仪

• 批准号: 434962735
• 金额: --
• 依托单位: Christian-Albrechts-Universität zu Kiel
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/434962735?language=en
• 关键词: Multicolor; Cell; sorter

Biomedical clinical and basic research is increasingly concentrating on the exact molecular and functional characterization of individual cells or cell groups within complex biological systems and organs, both internationally and as in biomedical research in Kiel. In Kiel, the main focus is on inflammatory medicine and precision medicine. Kiel also has outstanding expertise in the field of sequencing and various -omics technologies. The application of these technologies to the molecular analysis of defined (single) cells or groups is to be optimized and intensified by sorting the cell types to be investigated. Especially in the immune system and within complex tissue samples (patients) this is a challenge and necessity which can be ideally addressed by modern flow cytometric methods. This requires technical equipment that allows high-resolution analysis of single cell within cell mixtures using a maximum number of parameters and simultaneously purifying defined cell populations or individual cells for subsequent functional or molecular analysis steps. No adequate equipment is currently available in Kiel (a 14-year-old, technically limited device is no longer maintained by the manufacturer and must be replaced). In order to meet the variable requirements at the Kiel site, the purchase of a multi-colour cell sorter with 4 lasers, up to 16 colour parameters and single cell deflection is essential. Multidimensional cell sorting and analysis procedures require high instrumental and personnel costs as well as specialized and constantly updated know-how. Therefore, the device is to be integrated into a newly created cytometry core facility at the Institute of Immunology.

生物医学临床和基础研究越来越多地集中在复杂的生物系统和器官中的单个细胞或细胞群的精确分子和功能表征上，无论是在国际上还是在基尔的生物医学研究中。在基尔，主要关注炎症医学和精准医学。基尔还在测序和各种组学技术领域拥有杰出的专业知识。这些技术在确定的（单个）细胞或组的分子分析中的应用将通过分选待研究的细胞类型来优化和加强。特别是在免疫系统和复杂的组织样本（患者）中，这是一个挑战和必要性，可以通过现代流式细胞术方法理想地解决。这需要技术设备，其允许使用最大数量的参数对细胞混合物中的单个细胞进行高分辨率分析，并同时纯化限定的细胞群或单个细胞用于随后的功能或分子分析步骤。基尔目前没有足够的设备（制造商不再维护一个14年的技术有限设备，必须更换）。为了满足基尔工厂的各种要求，购买一台多色细胞分选机至关重要，该分选机配备4个激光器、多达16个颜色参数和单个细胞偏转。多维细胞分选和分析程序需要高昂的仪器和人员成本以及专业和不断更新的专业知识。因此，该设备将被集成到免疫学研究所新创建的细胞计数核心设施中。