Biophotonics: Quantum dots for single molecule imaging in live cells

生物光子学：用于活细胞中单分子成像的量子点

• 批准号: 0620813
• 负责人: Sanford Simon
• 金额: $ 51.85万
• 依托单位: Rockefeller University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0620813&HistoricalAwards=false
• 关键词: Biophotonics; Quantum; dots; single; molecule

0620813SimonThe work in this proposal will optimize fluorophores for following single proteins in cells. Thiscould significantly enhance the ability to study the roles of proteins in many different cellularreactions. There are some organic fluorophores that can be monovalently linked to proteins.Unfortunately, they are not photostable enough to be used for many biological studies. Quantumdots have significantly greater brightness and photostability. However, they aggregate and aremultivalent in their linkages. This project will optimize the imaging techniques and designs ofquantum dots to reduce aggregation and ensure that their linkages to proteins are monovalent.

[06:20 . 813]西蒙：本提案中的工作将优化荧光团，以跟踪细胞中的单个蛋白质。这可以显著提高研究蛋白质在许多不同细胞反应中的作用的能力。有一些有机荧光团可以与蛋白质单价连接。不幸的是，它们的光稳定性不足以用于许多生物学研究。量子点具有更大的亮度和光稳定性。然而，它们聚集在一起，在它们的联系中是多价的。该项目将优化量子点的成像技术和设计，以减少聚集并确保它们与蛋白质的连接是单价的。