Biophotonics: Quantum dots for single molecule imaging in live cells

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0620813SimonThe work in this proposal will optimize fluorophores for following single proteins in cells. Thiscould significantly enhance the ability to study the roles of proteins in many different cellularreactions. There are some organic fluorophores that can be monovalently linked to proteins.Unfortunately, they are not photostable enough to be used for many biological studies. Quantumdots have significantly greater brightness and photostability. However, they aggregate and aremultivalent in their linkages. This project will optimize the imaging techniques and designs ofquantum dots to reduce aggregation and ensure that their linkages to proteins are monovalent.