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Small Achiral Ligands as Vibrational Circular Dichroism Probes of Active Sites in Metalloproteins

小非手性配体作为金属蛋白活性位点的振动圆二色性探针

基本信息

批准号：
0111402
负责人：
Laurence Nafie
金额：
$ 30.95万
依托单位：
Syracuse University
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2001
资助国家：
美国
起止时间：
2001-08-01 至 2005-12-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0111402&HistoricalAwards=false
关键词：
Small Achiral Ligands Vibrational Circular

项目摘要

Dr. Laurence A. Nafie of Syracuse University is funded for his research on small achiral ligands as vibrational circular dichroism (VCD) probes of active sites in metalloproteins by the Physical Chemistry Program of the Chemistry Division. Improved experimental measurements and theoretical calculations will be used to complete the developement of VCD as a sensitive probe of metal-ligand active-sites in metalloproteins. New information about the solution-state structure and conformaton of metalloprotein complexes will be generated, and new insight into the (1) low-lying electronic structure of associated metal centers, (2) protein pocket environments in metalloproteins, and (3) coupling of these structural elements to the vibrational modes observed in VCD spectra will be obtained. Such studies will positively impact associated vibrational measurement techniques such as IR absorption and resonance Raman scattering in metalloprotein complexes.The use of various spectroscopies to better understand protein structure and function is a long-standing goal of experimental physical chemistry. The vibrational circular dichroism studies of metalloproteins and associated complexes at Syracuse University will extend our structure-function understanding of metalloproteins, so important to energy metabolism in biological systems.

Dr. Laurence A.锡拉丘兹大学的纳菲因其对小非手性配体作为金属蛋白活性位点的振动圆二色性（VCD）探针的研究而获得化学系物理化学计划的资助。改进的实验测量和理论计算将用于完成VCD作为金属蛋白中金属配体活性位点的灵敏探针的开发。将产生有关金属蛋白复合物的溶液态结构和构象的新信息，并将获得对（1）相关金属中心的低位电子结构，（2）金属蛋白中的蛋白质口袋环境，以及（3）这些结构元素与VCD光谱中观察到的振动模式的耦合的新见解。这些研究将积极影响相关的振动测量技术，如红外吸收和共振拉曼散射在金属蛋白复合物。使用各种光谱，以更好地了解蛋白质的结构和功能是实验物理化学的长期目标。锡拉丘兹大学对金属蛋白及其相关复合物的振动圆二色性研究将扩展我们对金属蛋白结构-功能的理解，金属蛋白对生物系统中的能量代谢非常重要。