SGER: High Resolution Imaging of Cancer Cells with Light Scattering Spectroscopy

本探索性研究的总体目标是开发一种新的高分辨率光谱成像技术，该技术利用上皮组织近反向弹性散射的光，在细胞和亚细胞尺度上提取宏观组织组成和组织结构信息。具体目的如下：1。实验系统。开发一种实验性高分辨率成像系统，用于成像浑浊介质的浅层，该系统使用光谱和角度信息来研究组织幻影，细胞和亚细胞细胞器。发展分离单散射光和多散射光以及提取组织形态信息的实验技术。建模。建立数学模型，描述来自单个亚细胞细胞器、细胞和细胞外组织的光散射。识别亚细胞细胞器的光谱特征，并开发一个数学程序来提取这些细胞器的物理性质，如尺寸分布、折射率和吸收系数。实验研究。研究单个细胞、亚细胞细胞器和细胞单层的单次和多次散射的波长和角度依赖性。开发基于散射光空间分布的方法来获取关于表层组织的组织/无序程度的信息，并利用这些信息在细胞尺度上重建组织的结构图像。

0116833PerelmanThe overall objective of the proposed exploratory study is to develop a novel high resolution spectroscopic imaging technique that utilizes light, elastically scattered from epithelial tissues in near backward direction, to extract information about macroscopic tissue composition as well as tissue structure at the cellular and sub-cellular scale. The specific aims are as follows:1. Experimental system. Develop an experimental high resolution imaging system for imaging superficial layers of turbid media, which uses both spectral and angular information to study tissue phantoms, cells, and sub-cellular organelles. Develop experimental techniques capable of separating single scattered and multiply scattered light and extracting morphological information from tissue.2. Modeling. Develop mathematical models that describe light scattering from individual sub-cellular organelles, cells and extra-cellular tissues. Identify spectroscopic signatures of sub-cellular organelles, and develop a mathematical procedure for extracting physical properties of those organelles, such as size distribution, refractive indices and absorption coefficients.3. Experimental studies. Study wavelength and angular dependence of single and multiple scattering from individual cells, sub-cellular organelles, and cell monolayers. Develop methods based on spatial distribution of scattered light to obtain information about degree of organization/disorganization of superficial tissues and use this information to reconstruct structural images of tissue on a cellular scale.