Arabidopsis 2010: Phenylpropanoid Pathway Networks: An Integrated Approach to Establishing Protein/Enzyme Function in Arabidopsis and their Associated Networks

这个拟南芥2010计划的目标是建立248个拟南芥酶和蛋白质的生理功能，这些酶和蛋白质被认为参与了各种苯丙酸-乙酸代谢网络。 有两个主要目标：鉴定与类苯丙素偶联/聚合相关的网络（例如，导致木质素、木脂素、木栓质、孢粉素等），包括这些酶/蛋白质的功能。 第二个目标是精确地识别存在于拟南芥中的参与苯丙氨酸转化为单木质素的不同网络。 在这两个目标中，功能将在体外证明，我们也将建立，这是真正的生理功能，通过展示时间和空间相关性与参与的代谢途径networks.This工作的片段将因此定义的各种苯丙素自由基的组织自由基耦合和相关的代谢过程中拟南芥通过其整个生命周期。 对科学界的好处将包括通过与拟南芥网站链接的网站迅速传播结果（在发表之前），并根据需要提供研究材料（基因、构建体、重组蛋白、转基因和突变植物）。 另一个重要的好处将是在这些迄今为止困难的系统（如偶联/聚合），涉及大分子组装获得的新知识，并将获得新的见解。 对于那些最终不参与这些途径的酶/基因，代谢物谱分析将提供功能的线索，这也将被检查。此外，除了木质化，这项研究将揭示体内其他高度调节的自由基-自由基酚偶联系统的重要信息，包括：种皮的构建及其代谢物;在生成（加强）毛状体基质;形成栓化组织和加强花柄;生物合成孢粉素（花粉粒中非常稳定的成分）;加强角质化组织;通过羟基肉桂酸交联细胞壁碳水化合物（酚）偶联，在生产过多的防御化合物，并可能用于其他目的等待发现。 除了同行评审的期刊，我们的研究成果，信息和材料产生的这项研究将提供给公共数据库，定期更新（每月）的基础上，通过发布数据/信息在一个专门的网站（http：//ibc.wsu.edu/lewislab/nsf/index.html），将连接到拟南芥网络网站相关的项目。 这些信息将包括：本实验室构建的重组蛋白表达载体;基因鉴定和基因功能分析;动力学数据结果;基因表达谱;代谢产物和木质素分析;原位杂交数据;和光学显微镜文件。

The goal of this Arabidopsis 2010 Project is to establish the physiological function of 248 Arabidopsis enzymes and proteins presumed to be involved in various networks of phenylpropanoid-acetate metabolism. There are two main objectives: identifying networks associated with phenylpropanoid coupling/polymerization (e.g. leading to lignins, lignans, suberins, sporopollenins, etc.), including how these enzymes/proteins function. The second objective is to precisely identify the different networks that exist in Arabidopsis that are involved in the conversion of phenylalanine through to the monolignols. In both objectives, functions will be demonstrated in vitro, and we will also establish that this is the true physiological function by demonstrating temporal and spatial correlation with the segments of the metabolic pathway networks involved.This work will thus define the organization of the various phenylpropanoid radical-radical coupling and related metabolic processes in Arabidopsis through its entire life cycle. The benefits to the scientific community will include rapid dissemination of results (prior to publication) through a website linked to the Arabidopsis sites, and the provision of research materials (genes, constructs, recombinant proteins, transgenic and mutant plants) as needed. Another important benefit will be the new knowledge gained on these hitherto difficult systems (e.g. coupling/polymerization) involving macromolecular assemblies, and the new insights that will be gained. For enzymes/genes chosen that are ultimately not involved in these pathways, it is considered that metabolite profiling will provide a clue as to function, and this will then be examined also.Further, in addition to lignification, this study will shed important light on other highly regulated radical-radical phenolic coupling systems in vivo including: the construction of seed coats and metabolites therein; in generating the (strengthened) matrix of trichomes; in forming suberized tissue and in strengthening flower stalks; in biosynthesizing sporopollenin (a remarkably stable component of pollen grains); in reinforcing cutinized tissue; in cross-linking cell wall carbohydrates through hydroxycinnamic acid (phenolic) coupling, in producing a plethora of defense compounds and presumably for other purposes awaiting discovery. In addition to peerreviewed journals, our research findings, information and materials generated by this research will be made available to public databases, updated periodically on a (monthly) basis, by posting data/information on a dedicated website (http://ibc.wsu.edu/lewislab/nsf/index.html) that will be linked up to the Arabidopsis network sites relevant to the project. This information will include: recombinant protein expression vectors constructed by our laboratory; gene identification and gene function analysis; kinetic data results; gene expression profiles; metabolite and lignin analysis; in situ hybridization data; and light microscopy documentation.