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US-Spain Cooperative Research: Designed Interfacial Assembly of Redox Active Enzymes and Recognition Layers for Biosensor Applications

美国-西班牙合作研究：为生物传感器应用设计氧化还原活性酶和识别层的界面组装

基本信息

批准号：
0122759
负责人：
Hector Abruna
金额：
$ 1.9万
依托单位：
Cornell University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2002
资助国家：
美国
起止时间：
2002-02-15 至 2004-01-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0122759&HistoricalAwards=false
关键词：
US Spain Cooperative Research Designed

项目摘要

0122759AbrunaThis two-year award for US-Spain collaboration on enzyme immobilization methodologies giving rise to oriented layers of enzymes leading to amperometric biosensor applications involves Hector Abruna and students at Cornell University and Encarnacion Lorenzo at the Universidad Autonoma of Madrid in Spain. Enzymes represent some of the most sophisticated catalysts known. Immobilization of active enzymes onto solid substrates can be used to generate sensors that exploit these features. Immobilization approaches tend to alter, if not destroy, an enzyme's activity, there is interest in new enzyme immobilization methodologies that will allow for the oriented immobilization of the enzyme layer while retaining enzymatic activity.The US researchers bring to this collaboration expertise in electrochemical techniques and in the synthesis and characterization of transition metal complexes. This is complemented by Spanish expertise in the preparation and characterization of a variety of sensors and in their application to the analysis of pesticides in foods and in environmental samples. This work, coupling diverse aspects of chemistry and biology, will be of interest and utility in environmental studies and monitoring of pollutants and in biotechnological applications.

0122759 Abruna这一为期两年的奖项是针对美国和西班牙在酶固定化方法上的合作，该方法产生了导致电流型生物传感器应用的酶定向层，涉及康奈尔大学的Hector Abruna和学生以及西班牙马德里自治大学的Encarnacion Lorenzo。酶代表了一些已知的最复杂的催化剂。将活性酶固定到固体基质上可用于产生利用这些特征的传感器。固定化方法往往会改变，如果不是破坏，酶的活性，有兴趣在新的酶固定化方法，将允许定向固定的酶层，同时保留酶的活性。美国研究人员带来了电化学技术和过渡金属配合物的合成和表征的专业知识，这一合作。西班牙在各种传感器的制备和特性鉴定方面以及在将其应用于食品和环境样品中农药分析方面的专门知识对此予以补充。这项工作，耦合化学和生物学的各个方面，将在环境研究和监测污染物和生物技术应用的兴趣和效用。