U.S.-Germany Cooperative Research: Effect of Thyroid Endocrine Disruptors on Gene Expression, Thyroid Homeostasis, and Reproduction in Amphibians and Fish

美德合作研究：甲状腺内分泌干扰物对两栖动物和鱼类基因表达、甲状腺稳态和繁殖的影响

• 批准号: 0129059
• 负责人: Christopher Theodorakis
• 金额: $ 1.6万
• 依托单位: Texas Tech University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-15 至 2005-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0129059&HistoricalAwards=false
• 关键词: U.S.; Germany; Cooperative; Research; Effect

0129059TheodorakisThis award supports Chrostopher Theodorakis and students from Texas Tech University in a collaboration with Rupert Simon of the National Research Center for Environment and Health in Neuherberg, Germany. The aim of the international project is to study the effect of thyroid-disrupting substance on molecular markers. The ecological relevance of these responses will be assessed in laboratory exposure experiments with zebrafish and African clawed frogs as fish and amphibian model species. The German group can provide samples of zebrafish exposed to selected pollutants, analyze them for thyroid hormones and iodinated compounds in tissue and serum samples, and determine exposure concentrations of organic contaminants in water and biological samples. The group at Texas Tech will expose zebrafish and African clawed frogs to perchlorate and analyze thyroid histology, analyze differential gene expression, and perform analysis of perchlorate in water and tissues. Results of the collaboration will develop new protocols for endocrine disrupting potential assessments of industrial and environmental chemicals. The work plan provides for extensive participation by graduate students in the international travel and research.

0129059Theodorakis该奖项支持德克萨斯理工大学的christopher Theodorakis和学生与德国纽赫伯格国家环境与健康研究中心的Rupert Simon的合作。国际项目的目的是研究甲状腺干扰物质对分子标记物的影响。这些反应的生态相关性将在以斑马鱼和非洲爪蛙作为鱼类和两栖动物模型物种的实验室暴露实验中进行评估。这个德国小组可以提供斑马鱼暴露于选定污染物的样本，分析组织和血清样本中的甲状腺激素和碘化化合物，并确定水和生物样本中有机污染物的暴露浓度。德克萨斯理工大学的研究小组将把斑马鱼和非洲爪蛙暴露在高氯酸盐中，分析甲状腺组织学，分析差异基因表达，并对水和组织中的高氯酸盐进行分析。合作的结果将为工业和环境化学品的内分泌干扰潜力评估制定新的议定书。工作计划规定研究生广泛参与国际旅行和研究。