U.S.-Germany Cooperative Research: Thermodynamic and Proteolytic Stability of Proteins Containing Non-Natural Modules

0129163 Raines该奖项支持Ronald Raines和威斯康星大学麦迪逊分校的学生与德国莱比锡大学生物化学和生物技术系的Renate Ulbrich-Hofmann合作。该国际项目的目标是使用内含子介导的蛋白质连接来创造核糖核酸酶A的半合成变种，这些变种将由美国小组生产，并用德国小组建立的方法研究其热力学和蛋白质分解稳定性。这些结果将为蛋白质折叠途径和蛋白质稳定性的基本基础提供新的见解，并最终导致创造出具有理想特性的新蛋白质。该工作计划允许研究生广泛参与国际旅行和研究。

0129163RainesThis award supports Ronald Raines and students from the University of Wisconsin-Madison in a collaboration with Renate Ulbrich-Hofmann of the Department of Biochemistry and Biotechnology at the University of Leipzig, Germany. The aim of the international project is to use intein-mediated protein ligation to create semisynthetic variants of ribonuclease A. These variants will be produced by the US group and studied with respect to their thermodynamic and proteolytic stability by methods established in the German group. The results will provide new insight into protein-folding pathways and the fundamental basis for protein stability, and ultimately lead to the creation of new proteins with desirable properties. The work plan provides for extensive participation by graduate students in the international travel and research.