U.S.-Germany Cooperative Research: The Biochemical Function of Basonuclin in RNA Polymerase Transcription

0202120Tseng该奖项支持Hung Tseng和宾夕法尼亚大学的一名学生与德国海德堡德国癌症研究中心分子细胞生物学部门的Ingrid Grummt合作。该项目将专注于Basonuclin的生化功能，Basonuclin是一种锌指蛋白，也是rRNA基因的细胞型特异性转录调节因子。美国和德国的合作将使用生化提纯、免疫学检测以及体外和体内转录试验来研究Basonuclin在聚合酶转录中的作用。所有的细胞都需要rRNA转录，但当蛋白质合成的速度在增殖和蛋白质积累中增加时，rRNA的增强尤为必要。Basonuclin作用的一个新方面是它是细胞类型特异性的，这使得它不同于所有先前描述的聚合酶I转录因子。美方将有关Basonuclin和试剂的最新知识带到合作中。Grummt博士的实验室是世界上RNA聚合酶I研究的领先团队之一。该实验室将提供生化纯化和分析聚合酶转录复合体的专业知识和最先进的设备。这一合作将有助于使德国和美国研究小组之间的合作关系制度化。

0202120TsengThis award supports Hung Tseng and a student from University of Pennsylvania in a collaboration with Ingrid Grummt of the Section for Molecular Cell Biology at the German Cancer Research Center in Heidelberg, Germany. The project will focus on the biochemical function of basonuclin, a zinc-finger protein and a cell-type specific transcription regulator for the rRNA genes. The US-German collaboration will use biochemical purification, immunological detection, and in vitro and in vivo transcription assays to investigate the role of basonuclin in polymerase transcription. All cell require rRNA transcription, but its enhancement is particularly necessary when the rate of protein synthesis increases in proliferation and protein accumulation. One of the novel aspects of the action of basonuclin is the it is cell-type specific, which makes it different from all previously described polymerase I transcription factors. The US side brings current knowledge about basonuclin and reagents to the collaboration. The laboratory of Dr. Grummt is one of the leading groups in the world in RNA polymerase I research. That lab will provide expertise and state-of-the-art equipment in biochemical purification and analysis of the polymerase transcription complex. This collaboration will help institutionalize the collaborative relationship between the German and US research groups.