Analysis of Speciation Mechanisms in an Adaptive Radiation

自适应辐射中的形态机制分析

• 批准号: 0129130
• 负责人: Scott Hodges
• 金额: $ 4.5万
• 依托单位: University of California-Santa Barbara
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2004-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0129130&HistoricalAwards=false
• 关键词: Analysis; Speciation; Mechanisms; Adaptive; Radiation

Adaptive radiations have long been recognized as prime examples of natural selection and speciation. To understand the processes that bring about these radiations requires a phylogenetic reconstruction of the relationships among the species. However, adaptive radiations usually occur extremely rapidly and therefore traditional methods of phylogenetic reconstruction often fail. The proposed research will investigate whether DNA sequencing of the 3'-untranslated region of multiple genes will provide the data necessary for the phylogenetic reconstruction of a particularly rapid adaptive radiation, that in the columbine genus Aquilegia. After sequencing the 3' ends of expressed genes, specific PCR primers will be designed to amplify both coding and non-coding gene regions. These gene regions will then be sequenced for multiple species of Aquilegia and a phylogenetic reconstruction will be made. Understanding the processes that generate bio-diversity is essential to understanding how bio-diversity can be maintained. Adaptive radiations represent instances of remarkable diversification yet without information on the evolutionary relationships of their species, little progress can be made on understanding their origin. The proposed research will investigate a technique that would be applicable to reconstructing relationships for a wide range of taxa.

长期以来，适应性辐射一直被认为是自然选择和物种形成的主要例子。为了理解这些辐射的过程，需要对物种之间的关系进行系统发育重建。然而，适应性辐射通常发生得非常迅速，因此传统的系统发育重建方法往往失败。拟议的研究将调查多个基因的3 '-非翻译区的DNA测序是否将提供必要的数据，用于重建一种特别快速的适应性辐射，即在Columbine属Aquilegia中的系统发育。 在对表达基因的3'端测序后，将设计特异性PCR引物以扩增编码和非编码基因区。这些基因区域，然后将测序多个物种的Aquilegia和系统发育重建将。了解产生生物多样性的过程对于了解如何维持生物多样性至关重要。适应性辐射代表了显着的多样化的例子，但没有关于其物种进化关系的信息，在了解其起源方面几乎没有进展。拟议的研究将调查一种技术，将适用于重建广泛的类群的关系。