Meiotic Recombination and DNA Repair in Drosophila

细胞修复DNA链间交联的机制在很大程度上仍然未知。 在哺乳动物中，核苷酸切除修复核酸内切酶XPF-Ercc 1在链间交联修复中起关键作用。 这种功能在果蝇XPF同源物MEI-9中是保守的。 该项目涉及MEI-9与新鉴定的交联修复蛋白MUS 312之间的相互作用。 mus 312突变体的遗传分析将更好地了解哪些修复和重组途径需要mus 312，以及mus 312和mei-9在相同途径中的作用程度。 将产生mus 312的其他等位基因以更好地理解MUS 312蛋白。 mei-9和mus 312也是产生减数分裂交换的关键，这一功能也将在遗传学和分子学上进行研究。DNA受到内部和外部因素的持续攻击;几种化疗药物通过在DNA中产生链间交联来发挥作用。 正常细胞修复这种损伤的机制尚不清楚。 目前的研究旨在了解两种关键蛋白MEI-9和MUS 312对这种修复的贡献。 这些蛋白质相互作用，在果蝇中进行修复过程。

The mechanisms by which cells repair DNA interstrand crosslinks remain largely unknown. In mammals, the nucleotide excision repair endonuclease XPF-Ercc1 plays a key role in interstrand crosslink repair. This function is conserved in the Drosophila XPF homolog MEI-9. This project concerns the interaction between MEI-9 and a newly identified crosslink repair protein, MUS312. Genetic analyses of mus312 mutants will be done to better understand which repair and recombination pathways require mus312, and to what extent mus312 and mei-9 act in the same pathways. Additional alleles of mus312 will be generated to better understand the MUS312 protein. Both mei-9 and mus312 are also essential for generating meiotic crossovers, and this function will also be studied genetically and molecularly.DNA is under constant attack by both internal and external agents; several chemotherapy agents exert their effects by creating interstrand crosslinks in DNA. The mechanisms by which normal cells repair this type of damage are unknown. The present research seeks to understand the contribution to this repair of two key proteins, MEI-9 and MUS312. These proteins interact physically to carry out this repair process in Drosophila melanogaster.